Measurement system for simultaneous observation of myosin V chemical and mechanical events Export

@article{citeulike:3922029, abstract = {Myosin V is an actin-based processive molecular motor driven by the chemical energy of ATP hydrolysis. Although the chemo-mechanical coupling in processive movement has been postulated by separate structural, mechanical and biochemical studies, no experiment has been able to directly test these conclusions. Therefore the relationship between ATP-turnover and force generation remains unclear. Currently, the most direct method to measure the chemo-mechanical coupling in processive motors is to simultaneously observe ATP-turnover cycles and displacement at the single molecule level. In this study, we developed a simultaneous measurement system suitable for mechanical and chemical assays of myosin V in order to directly elucidate its chemo-mechanical coupling.}, author = {Komori, T. and Nishikawa, S. and Ariga, T. and Iwane, A. and Yanagida, T.}, citeulike-article-id = {3922029}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.biosystems.2008.04.004}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0303264708000737}, doi = {10.1016/j.biosystems.2008.04.004}, issn = {03032647}, journal = {Biosystems}, keywords = {fluorescent-nucleotide, myosin, myosin-v}, month = {July}, number = {1-2}, pages = {48--57}, posted-at = {2009-01-21 22:59:37}, priority = {1}, title = {Measurement system for simultaneous observation of myosin V chemical and mechanical events}, url = {http://dx.doi.org/10.1016/j.biosystems.2008.04.004}, volume = {93}, year = {2008} }

TY - JOUR ID - citeulike:3922029 L3 - citeulike-article-id:3922029 N2 - Myosin V is an actin-based processive molecular motor driven by the chemical energy of ATP hydrolysis. Although the chemo-mechanical coupling in processive movement has been postulated by separate structural, mechanical and biochemical studies, no experiment has been able to directly test these conclusions. Therefore the relationship between ATP-turnover and force generation remains unclear. Currently, the most direct method to measure the chemo-mechanical coupling in processive motors is to simultaneously observe ATP-turnover cycles and displacement at the single molecule level. In this study, we developed a simultaneous measurement system suitable for mechanical and chemical assays of myosin V in order to directly elucidate its chemo-mechanical coupling. IS - 1-2 JF - Biosystems SN - 03032647 EP - 57 TI - Measurement system for simultaneous observation of myosin V chemical and mechanical events VL - 93 SP - 48 KW - fluorescent-nucleotide KW - myosin KW - myosin-v AU - Komori, T AU - Nishikawa, S AU - Ariga, T AU - Iwane, A AU - Yanagida, T PY - 2008/07// UR - http://dx.doi.org/10.1016/j.biosystems.2008.04.004 ER -