Automated design of synthetic ribosome binding sites to control protein expression. Export

@article{citeulike:5891194, abstract = {Microbial engineering often requires fine control over protein expression--for example, to connect genetic circuits or control flux through a metabolic pathway. To circumvent the need for trial and error optimization, we developed a predictive method for designing synthetic ribosome binding sites, enabling a rational control over the protein expression level. Experimental validation of >100 predictions in Escherichia coli showed that the method is accurate to within a factor of 2.3 over a range of 100,000-fold. The design method also correctly predicted that reusing identical ribosome binding site sequences in different genetic contexts can result in different protein expression levels. We demonstrate the method's utility by rationally optimizing protein expression to connect a genetic sensor to a synthetic circuit. The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems.}, author = {Salis, Howard M. and Mirsky, Ethan A. and Voigt, Christopher A.}, citeulike-article-id = {5891194}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.1568}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.1568}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19801975}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19801975}, day = {04}, doi = {10.1038/nbt.1568}, issn = {1546-1696}, journal = {Nature biotechnology}, keywords = {automated\_design, bioinformatics, forward\_engineering, genetic\_systems, protein\_expression\_level, ribosome\_binding\_site, sequences}, month = {October}, number = {10}, pages = {946--950}, posted-at = {2009-10-20 22:56:55}, priority = {2}, publisher = {Nature Publishing Group}, title = {Automated design of synthetic ribosome binding sites to control protein expression.}, url = {http://dx.doi.org/10.1038/nbt.1568}, volume = {27}, year = {2009} }

TY - JOUR ID - citeulike:5891194 L3 - citeulike-article-id:5891194 N2 - Microbial engineering often requires fine control over protein expression--for example, to connect genetic circuits or control flux through a metabolic pathway. To circumvent the need for trial and error optimization, we developed a predictive method for designing synthetic ribosome binding sites, enabling a rational control over the protein expression level. Experimental validation of >100 predictions in Escherichia coli showed that the method is accurate to within a factor of 2.3 over a range of 100,000-fold. The design method also correctly predicted that reusing identical ribosome binding site sequences in different genetic contexts can result in different protein expression levels. We demonstrate the method's utility by rationally optimizing protein expression to connect a genetic sensor to a synthetic circuit. The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems. IS - 10 JF - Nature biotechnology SN - 1546-1696 EP - 950 TI - Automated design of synthetic ribosome binding sites to control protein expression. PB - Nature Publishing Group VL - 27 SP - 946 KW - automated_design KW - bioinformatics KW - forward_engineering KW - genetic_systems KW - protein_expression_level KW - ribosome_binding_site KW - sequences AU - Salis, Howard AU - Mirsky, Ethan AU - Voigt, Christopher PY - 2009/10/04/ UR - http://dx.doi.org/10.1038/nbt.1568 ER -