Induction of protein-protein interactions in live cells using light Export

@article{citeulike:5917014, abstract = {Protein-protein interactions are essential for many cellular processes. We have developed a technology called light-activated dimerization (LAD) to artificially induce protein hetero- and homodimerization in live cells using light. Using the FKF1 and GIGANTEA (GI) proteins of Arabidopsis thaliana, we have generated protein tags whose interaction is controlled by blue light. We demonstrated the utility of this system with LAD constructs that can recruit the small G-protein Rac1 to the plasma membrane and induce the local formation of lamellipodia in response to focal illumination. We also generated a light-activated transcription factor by fusing domains of GI and FKF1 to the DNA binding domain of Gal4 and the transactivation domain of VP16, respectively, showing that this technology is easily adapted to other systems. These studies set the stage for the development of light-regulated signaling molecules for controlling receptor activation, synapse formation and other signaling events in organisms.}, author = {Yazawa, Masayuki and Sadaghiani, Amir M. and Hsueh, Brian and Dolmetsch, Ricardo E.}, citeulike-article-id = {5917014}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.1569}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.1569}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19801976}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19801976}, day = {04}, doi = {10.1038/nbt.1569}, issn = {1087-0156}, journal = {Nature Biotechnology}, keywords = {biotechnology, dna\_binding\_domain, illumination, protein\_protein\_interactions}, month = {October}, number = {10}, pages = {941--945}, posted-at = {2009-10-20 22:41:34}, priority = {2}, publisher = {Nature Publishing Group}, title = {Induction of protein-protein interactions in live cells using light}, url = {http://dx.doi.org/10.1038/nbt.1569}, volume = {27}, year = {2009} }

TY - JOUR ID - citeulike:5917014 L3 - citeulike-article-id:5917014 N2 - Protein-protein interactions are essential for many cellular processes. We have developed a technology called light-activated dimerization (LAD) to artificially induce protein hetero- and homodimerization in live cells using light. Using the FKF1 and GIGANTEA (GI) proteins of Arabidopsis thaliana, we have generated protein tags whose interaction is controlled by blue light. We demonstrated the utility of this system with LAD constructs that can recruit the small G-protein Rac1 to the plasma membrane and induce the local formation of lamellipodia in response to focal illumination. We also generated a light-activated transcription factor by fusing domains of GI and FKF1 to the DNA binding domain of Gal4 and the transactivation domain of VP16, respectively, showing that this technology is easily adapted to other systems. These studies set the stage for the development of light-regulated signaling molecules for controlling receptor activation, synapse formation and other signaling events in organisms. IS - 10 JF - Nature Biotechnology SN - 1087-0156 EP - 945 TI - Induction of protein-protein interactions in live cells using light PB - Nature Publishing Group VL - 27 SP - 941 KW - biotechnology KW - dna_binding_domain KW - illumination KW - protein_protein_interactions AU - Yazawa, Masayuki AU - Sadaghiani, Amir AU - Hsueh, Brian AU - Dolmetsch, Ricardo PY - 2009/10/04/ UR - http://dx.doi.org/10.1038/nbt.1569 ER -