Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous cultures. Export

@article{delneri2007, abstract = {Using competition experiments in continuous cultures grown in different nutrient environments (glucose limited, ammonium limited, phosphate limited and white grape juice), we identified genes that show haploinsufficiency phenotypes (reduced growth rate when hemizygous) or haploproficiency phenotypes (increased growth rate when hemizygous). Haploproficient genes (815, 1,194, 733 and 654 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) frequently show that phenotype in a specific environmental context. For instance, genes encoding components of the ubiquitination pathway or the proteasome show haploproficiency in nitrogen-limited conditions where protein conservation may be beneficial. Haploinsufficiency is more likely to be observed in all environments, as is the case with genes determining polar growth of the cell. Haploproficient genes seem randomly distributed in the genome, whereas haploinsufficient genes (685, 765, 1,277 and 217 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) are over-represented on chromosome III. This chromosome determines a yeast's mating type, and the concentration of haploinsufficient genes there may be a mechanism to prevent its loss.}, author = {Delneri, Daniela and Hoyle, David C. and Gkargkas, Konstantinos and Cross, Emma J. M. and Rash, Bharat and Zeef, Leo and Leong, Hui-Sun and Davey, Hazel M. and Hayes, Andrew and Kell, Douglas B. and Griffith, Gareth W. and Oliver, Stephen G.}, citeulike-article-id = {2175466}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng.2007.49}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng.2007.49}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18157128}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18157128}, day = {23}, doi = {10.1038/ng.2007.49}, issn = {1546-1718}, journal = {Nature Genetics}, keywords = {haploinsufficiency, mdb-thesis, metabolic-control-analysis}, month = {January}, number = {1}, pages = {113--117}, posted-at = {2009-06-10 11:33:52}, priority = {0}, publisher = {Nature Publishing Group}, title = {Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous cultures.}, url = {http://dx.doi.org/10.1038/ng.2007.49}, volume = {40}, year = {2008} }

TY - JOUR ID - delneri2007 L3 - citeulike-article-id:2175466 N2 - Using competition experiments in continuous cultures grown in different nutrient environments (glucose limited, ammonium limited, phosphate limited and white grape juice), we identified genes that show haploinsufficiency phenotypes (reduced growth rate when hemizygous) or haploproficiency phenotypes (increased growth rate when hemizygous). Haploproficient genes (815, 1,194, 733 and 654 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) frequently show that phenotype in a specific environmental context. For instance, genes encoding components of the ubiquitination pathway or the proteasome show haploproficiency in nitrogen-limited conditions where protein conservation may be beneficial. Haploinsufficiency is more likely to be observed in all environments, as is the case with genes determining polar growth of the cell. Haploproficient genes seem randomly distributed in the genome, whereas haploinsufficient genes (685, 765, 1,277 and 217 in glucose-limited, ammonium-limited, phosphate-limited and white grape juice environments, respectively) are over-represented on chromosome III. This chromosome determines a yeast's mating type, and the concentration of haploinsufficient genes there may be a mechanism to prevent its loss. IS - 1 JF - Nature Genetics SN - 1546-1718 EP - 117 TI - Identification and characterization of high-flux-control genes of yeast through competition analyses in continuous cultures. PB - Nature Publishing Group VL - 40 SP - 113 KW - haploinsufficiency KW - mdb-thesis KW - metabolic-control-analysis AU - Delneri, Daniela AU - Hoyle, David AU - Gkargkas, Konstantinos AU - Cross, Emma AU - Rash, Bharat AU - Zeef, Leo AU - Leong, Hui-Sun AU - Davey, Hazel AU - Hayes, Andrew AU - Kell, Douglas AU - Griffith, Gareth AU - Oliver, Stephen PY - 2008/01/23/ UR - http://dx.doi.org/10.1038/ng.2007.49 ER -