Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity. Export

@article{citeulike:3443549, abstract = {The minichromosome maintenance (MCM) proteins are thought to function as the replicative helicases in archaea. In most archaeal species studied, the interaction between MCM and the initiator protein, Cdc6, inhibits helicase activity. To date, the only exception is the helicase and Cdc6 proteins from the archaeon Thermoplasma acidophilum. It was previously shown that when the Cdc6 protein interacts with MCM it substantially stimulates helicase activity. It is shown here that the mechanism by which the Cdc6 protein stimulates helicase activity is by stimulating the ATPase activity of MCM. Also, through the use of site-specific substitutions, and truncated and chimeric proteins, it was shown that an intact Cdc6 protein is required for this stimulation. ATP binding and hydrolysis by the Cdc6 protein is not needed for the stimulation. The data suggest that binding of Cdc6 protein to MCM protein changes the structure of the helicase, enhancing the catalytic hydrolysis of ATP and helicase activity.}, author = {Haugland, G. T. and Sakakibara, N. and Pey, A. L. and Rollor, C. R. and Birkeland, N. K. and Kelman, Z.}, citeulike-article-id = {3443549}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18757887}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18757887}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {cdc6}, month = {October}, number = {17}, pages = {5602--5609}, posted-at = {2008-10-23 14:27:41}, priority = {2}, title = {Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18757887}, volume = {36}, year = {2008} }

TY - JOUR ID - citeulike:3443549 L3 - citeulike-article-id:3443549 N2 - The minichromosome maintenance (MCM) proteins are thought to function as the replicative helicases in archaea. In most archaeal species studied, the interaction between MCM and the initiator protein, Cdc6, inhibits helicase activity. To date, the only exception is the helicase and Cdc6 proteins from the archaeon Thermoplasma acidophilum. It was previously shown that when the Cdc6 protein interacts with MCM it substantially stimulates helicase activity. It is shown here that the mechanism by which the Cdc6 protein stimulates helicase activity is by stimulating the ATPase activity of MCM. Also, through the use of site-specific substitutions, and truncated and chimeric proteins, it was shown that an intact Cdc6 protein is required for this stimulation. ATP binding and hydrolysis by the Cdc6 protein is not needed for the stimulation. The data suggest that binding of Cdc6 protein to MCM protein changes the structure of the helicase, enhancing the catalytic hydrolysis of ATP and helicase activity. IS - 17 JF - Nucleic acids research SN - 1362-4962 EP - 5609 TI - Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity. VL - 36 SP - 5602 KW - cdc6 AU - Haugland, GT AU - Sakakibara, N AU - Pey, AL AU - Rollor, CR AU - Birkeland, NK AU - Kelman, Z PY - 2008/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/18757887 ER -