The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum. Export

@article{citeulike:3451454, abstract = {The social amoeba Dictyostelium discoideum contains only one aromatic amino acid hydroxylase (AAAH) gene compared to at least three in metazoans. As shown in this work this gene codes for a phenylalanine hydroxylase (DictyoPAH) and phylogenetic analysis places this enzyme close to the precursor AAAHs, aiding to define the evolutionary history of the AAAH family. DictyoPAH shows significant similarities to other eukaryote PAH, but it exhibits higher activity with tetrahydrodictyopterin (DH(4)) than with tetrahydrobiopterin (BH(4)) as cofactor. DH(4) is an abundant tetrahydropterin in D. discoideum while BH(4) is the natural cofactor of the AAAHs in mammals. Moreover, DictyoPAH is devoid of the characteristic regulatory mechanisms of mammalian PAH such as positive cooperativity for L-Phe and activation by preincubation with the substrate. Analysis of the few active site substitutions between DictyoPAH and mammalian PAH, including mutant expression analysis, reveals potential structural determinants for allosteric regulation.}, author = {Siltberg-Liberles, Jessica and Steen, Ida Helene H. and Svebak, Randi M M. and Martinez, Aurora}, citeulike-article-id = {3451454}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.gene.2008.09.005}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0378111908004393}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18835579}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18835579}, day = {16}, doi = {10.1016/j.gene.2008.09.005}, issn = {0378-1119}, journal = {Gene}, keywords = {dictyostelium, evolution, pah}, month = {September}, posted-at = {2008-10-26 15:37:02}, priority = {2}, title = {The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum.}, url = {http://dx.doi.org/10.1016/j.gene.2008.09.005}, year = {2008} }

TY - JOUR ID - citeulike:3451454 L3 - citeulike-article-id:3451454 N2 - The social amoeba Dictyostelium discoideum contains only one aromatic amino acid hydroxylase (AAAH) gene compared to at least three in metazoans. As shown in this work this gene codes for a phenylalanine hydroxylase (DictyoPAH) and phylogenetic analysis places this enzyme close to the precursor AAAHs, aiding to define the evolutionary history of the AAAH family. DictyoPAH shows significant similarities to other eukaryote PAH, but it exhibits higher activity with tetrahydrodictyopterin (DH(4)) than with tetrahydrobiopterin (BH(4)) as cofactor. DH(4) is an abundant tetrahydropterin in D. discoideum while BH(4) is the natural cofactor of the AAAHs in mammals. Moreover, DictyoPAH is devoid of the characteristic regulatory mechanisms of mammalian PAH such as positive cooperativity for L-Phe and activation by preincubation with the substrate. Analysis of the few active site substitutions between DictyoPAH and mammalian PAH, including mutant expression analysis, reveals potential structural determinants for allosteric regulation. JF - Gene SN - 0378-1119 TI - The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum. KW - dictyostelium KW - evolution KW - pah AU - Siltberg-Liberles, Jessica AU - Steen, Ida Helene AU - Svebak, Randi M AU - Martinez, Aurora PY - 2008/09/16/ UR - http://dx.doi.org/10.1016/j.gene.2008.09.005 ER -