Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation. Export

@article{citeulike:5008649, abstract = {Methylation of CpG islands associated with genes can affect the expression of the proximal gene, and methylation of non-associated CpG islands correlates to genomic instability. This epigenetic modification has been shown to be important in many pathologies, from development and disease to cancer. We report the development of a novel high-resolution microarray that detects the methylation status of over 25 000 CpG islands in the human genome. Experiments were performed to demonstrate low system noise in the methodology and that the array probes have a high signal to noise ratio. Methylation measurements between different cell lines were validated demonstrating the accuracy of measurement. We then identified alterations in CpG islands, both those associated with gene promoters, as well as non-promoter-associated islands in a set of breast and ovarian tumors. We demonstrate that this methodology accurately identifies methylation profiles in cancer and in principle it can differentiate any CpG methylation alterations and can be adapted to analyze other species.}, author = {Kamalakaran, Sitharthan and Kendall, Jude and Zhao, Xiaoyue and Tang, Chunlao and Khan, Sohail and Ravi, Kandasamy and Auletta, Theresa and Riggs, Michael and Wang, Yun and Helland, Aslaug and Naume, Bj{\o}rn and Dimitrova, Nevenka and B{\o}rresen-Dale, Anne-Lise L. and Hicks, Jim and Lucito, Robert}, citeulike-article-id = {5008649}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkp413}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19474344}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19474344}, day = {27}, doi = {10.1093/nar/gkp413}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {array, gene\_specific\_methylation, gwas, methodology, methylation}, month = {May}, posted-at = {2009-06-29 09:52:45}, priority = {2}, title = {Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation.}, url = {http://dx.doi.org/10.1093/nar/gkp413}, year = {2009} }

TY - JOUR ID - citeulike:5008649 L3 - citeulike-article-id:5008649 N2 - Methylation of CpG islands associated with genes can affect the expression of the proximal gene, and methylation of non-associated CpG islands correlates to genomic instability. This epigenetic modification has been shown to be important in many pathologies, from development and disease to cancer. We report the development of a novel high-resolution microarray that detects the methylation status of over 25 000 CpG islands in the human genome. Experiments were performed to demonstrate low system noise in the methodology and that the array probes have a high signal to noise ratio. Methylation measurements between different cell lines were validated demonstrating the accuracy of measurement. We then identified alterations in CpG islands, both those associated with gene promoters, as well as non-promoter-associated islands in a set of breast and ovarian tumors. We demonstrate that this methodology accurately identifies methylation profiles in cancer and in principle it can differentiate any CpG methylation alterations and can be adapted to analyze other species. JF - Nucleic acids research SN - 1362-4962 TI - Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation. KW - array KW - gene_specific_methylation KW - gwas KW - methodology KW - methylation AU - Kamalakaran, Sitharthan AU - Kendall, Jude AU - Zhao, Xiaoyue AU - Tang, Chunlao AU - Khan, Sohail AU - Ravi, Kandasamy AU - Auletta, Theresa AU - Riggs, Michael AU - Wang, Yun AU - Helland, Aslaug AU - Naume, Bjørn AU - Dimitrova, Nevenka AU - Børresen-Dale, Anne-Lise AU - Hicks, Jim AU - Lucito, Robert PY - 2009/05/27/ UR - http://dx.doi.org/10.1093/nar/gkp413 ER -