Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor. Export

@article{citeulike:2858830, abstract = {The ammonium-oxidizing microbial community was investigated in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor that was operated for about 1 year with high anaerobic ammonium oxidation activity (up to 0.8 kg NH(4)(+)-N m(-3) day(-1)). A Planctomycetales-specific 16S rRNA gene library was constructed to analyse the diversity of the anaerobic ammonium-oxidizing bacteria (AnAOB). Most of the specifically amplified sequences (15/16) were similar to each other (> 99\%) but were distantly related to all of the previously recognized sequences (< 94\%), with the exception of an unclassified anammox-related clone, KSU-1 (98\%). An ammonia monooxygenase (amoA) gene library was also analysed to investigate the diversity of 'aerobic' ammonium-oxidizing bacteria (AAOB) from the beta-Proteobacteria. Most of the amoA gene fragments (53/55) clustered in the Nitrosomonas europaea-Nitrosococcus mobilis group which has been reported to prevail under oxygen-limiting conditions. The quantitative results from real-time polymerase chain reaction (PCR) amplification showed that the dominant AnAOB comprised approximately 50\% of the total bacterial 16S rRNA genes in the reactor, whereas the AAOB of beta-Proteobacteria represented only about 3\%. A large fragment (4008 bp) of the rRNA gene cluster of the dominant AnAOB (AS-1) in this reactor sludge was sequenced and compared with sequences of other Planctomycetales including four anammox-related candidate genera. The partial sequence of hydrazine-oxidizing enzyme (hzo) of dominant AnAOB was also identified using new designed primers. Based on this analysis, we propose to tentatively name this new AnAOB Candidatus'Jettenia asiatica'.}, address = {Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China.}, author = {Quan, Zhe-Xue X. and Rhee, Sung-Keun K. and Zuo, Jian-E E. and Yang, Yang and Bae, Jin-Woo W. and Park, Ja Ryeong R. and Lee, Sung-Taik T. and Park, Yong-Ha H.}, citeulike-article-id = {2858830}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1462-2920.2008.01642.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18479446}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18479446}, day = {9}, doi = {10.1111/j.1462-2920.2008.01642.x}, issn = {1462-2920}, journal = {Environmental microbiology}, keywords = {16s, 23s, anammox, asiatica, hydrazine, hzo, jettenia, rrna}, month = {November}, number = {11}, pages = {3130--3139}, posted-at = {2008-06-03 12:38:13}, priority = {0}, title = {Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor.}, url = {http://dx.doi.org/10.1111/j.1462-2920.2008.01642.x}, volume = {10}, year = {2008} }

TY - JOUR ID - citeulike:2858830 L3 - citeulike-article-id:2858830 N2 - The ammonium-oxidizing microbial community was investigated in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor that was operated for about 1 year with high anaerobic ammonium oxidation activity (up to 0.8 kg NH(4)(+)-N m(-3) day(-1)). A Planctomycetales-specific 16S rRNA gene library was constructed to analyse the diversity of the anaerobic ammonium-oxidizing bacteria (AnAOB). Most of the specifically amplified sequences (15/16) were similar to each other (> 99%) but were distantly related to all of the previously recognized sequences (< 94%), with the exception of an unclassified anammox-related clone, KSU-1 (98%). An ammonia monooxygenase (amoA) gene library was also analysed to investigate the diversity of 'aerobic' ammonium-oxidizing bacteria (AAOB) from the beta-Proteobacteria. Most of the amoA gene fragments (53/55) clustered in the Nitrosomonas europaea-Nitrosococcus mobilis group which has been reported to prevail under oxygen-limiting conditions. The quantitative results from real-time polymerase chain reaction (PCR) amplification showed that the dominant AnAOB comprised approximately 50% of the total bacterial 16S rRNA genes in the reactor, whereas the AAOB of beta-Proteobacteria represented only about 3%. A large fragment (4008 bp) of the rRNA gene cluster of the dominant AnAOB (AS-1) in this reactor sludge was sequenced and compared with sequences of other Planctomycetales including four anammox-related candidate genera. The partial sequence of hydrazine-oxidizing enzyme (hzo) of dominant AnAOB was also identified using new designed primers. Based on this analysis, we propose to tentatively name this new AnAOB Candidatus'Jettenia asiatica'. IS - 11 JF - Environmental microbiology SN - 1462-2920 AD - Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China. EP - 3139 TI - Diversity of ammonium-oxidizing bacteria in a granular sludge anaerobic ammonium-oxidizing (anammox) reactor. VL - 10 SP - 3130 KW - 16s KW - 23s KW - anammox KW - asiatica KW - hydrazine KW - hzo KW - jettenia KW - rrna AU - Quan, Zhe-Xue AU - Rhee, Sung-Keun AU - Zuo, Jian-E AU - Yang, Yang AU - Bae, Jin-Woo AU - Park, Ja Ryeong AU - Lee, Sung-Taik AU - Park, Yong-Ha PY - 2008/11/09/ UR - http://dx.doi.org/10.1111/j.1462-2920.2008.01642.x ER -