Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis). Export

@article{citeulike:103073, abstract = {Sarcocystis neurona was isolated from the brain of a juvenile, male southern sea otter (Enhydra lutris nereis) suffering from CNS disease. Schizonts and merozoites in tissue sections of the otter's brain reacted with anti-S. neurona antiserum immunohistochemically. Development in cell culture was by endopolyogeny and mature schizonts were first observed at 3 days postinoculation. PCR of merozoite DNA using primer pairs JNB33/JNB54 and restriction enzyme digestion of the 1100 bp product with Dra I indicated the organism was S. neurona. Four of four interferon-gamma gene knockout mice inoculated with merozoites developed S. neurona-associated encephalitis. Antibodies to S. neurona but not Sarcocystis falcatula, Toxoplasma gondii, or Neospora caninum were present in the serum of inoculated mice. This is the first isolation of S. neurona from the brain of a non-equine host.}, address = {Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0342, USA. lindsayd@vt.edu}, author = {Lindsay, D. S. and Thomas, N. J. and Dubey, J. P.}, citeulike-article-id = {103073}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10779575}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10779575}, day = {24}, issn = {0020-7519}, journal = {Int J Parasitol}, keywords = {enhydra, epidemiology, exposure, lutris, molecular, neurona, pathogen, sarcocystis}, month = {April}, number = {5}, pages = {617--624}, posted-at = {2005-02-24 17:30:12}, priority = {4}, title = {Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis).}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10779575}, volume = {30}, year = {2000} }

TY - JOUR ID - citeulike:103073 L3 - citeulike-article-id:103073 N2 - Sarcocystis neurona was isolated from the brain of a juvenile, male southern sea otter (Enhydra lutris nereis) suffering from CNS disease. Schizonts and merozoites in tissue sections of the otter's brain reacted with anti-S. neurona antiserum immunohistochemically. Development in cell culture was by endopolyogeny and mature schizonts were first observed at 3 days postinoculation. PCR of merozoite DNA using primer pairs JNB33/JNB54 and restriction enzyme digestion of the 1100 bp product with Dra I indicated the organism was S. neurona. Four of four interferon-gamma gene knockout mice inoculated with merozoites developed S. neurona-associated encephalitis. Antibodies to S. neurona but not Sarcocystis falcatula, Toxoplasma gondii, or Neospora caninum were present in the serum of inoculated mice. This is the first isolation of S. neurona from the brain of a non-equine host. IS - 5 JF - Int J Parasitol SN - 0020-7519 AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0342, USA. lindsayd@vt.edu EP - 624 TI - Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis). VL - 30 SP - 617 KW - enhydra KW - epidemiology KW - exposure KW - lutris KW - molecular KW - neurona KW - pathogen KW - sarcocystis AU - Lindsay, DS AU - Thomas, NJ AU - Dubey, JP PY - 2000/04/24/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10779575 ER -