A Fluorescent Nanosensor for Apoptotic Cells Export

@article{citeulike:2415216, abstract = {Abstract: A biocompatible surface-functionalized nanoparticle was designed to sense phosphatidylserine exposed on apoptotic cells. We conjugated synthetic artificial phosphatidylserine binding ligands in a multivalent fashion onto magnetofluorescent nanoparticles. Our results show that (1) the synthetic nanoparticles bind to apoptotic cells, (2) there is excellent correlation with annexin V staining by microscopy, and (3) FACS analysis with nanoparticles allows the measurement of therapeutic apoptosis induction. The described nanomaterials should be useful for a variety of biomedical applications including in vivo imaging of apoptosis.}, address = {Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129}, author = {Quinti, L. and Weissleder, R. and Tung, C. H.}, citeulike-article-id = {2415216}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/nl0524694}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/nl0524694}, day = {8}, doi = {10.1021/nl0524694}, journal = {Nano Lett.}, keywords = {annexin-v, apoptosis, apoptotic-cells, ccne-grant, dextran-coated-aminated-clio, facs, flow-cytometry, fluorescence-activated-cell-sorting, fluorescent-microscopy, jurkat-t-cells, mit-mgh-ccne, necrosis}, month = {March}, number = {3}, pages = {488--490}, posted-at = {2008-02-22 20:04:00}, priority = {0}, title = {A Fluorescent Nanosensor for Apoptotic Cells}, url = {http://dx.doi.org/10.1021/nl0524694}, volume = {6}, year = {2006} }

TY - JOUR ID - citeulike:2415216 L3 - citeulike-article-id:2415216 N2 - Abstract: A biocompatible surface-functionalized nanoparticle was designed to sense phosphatidylserine exposed on apoptotic cells. We conjugated synthetic artificial phosphatidylserine binding ligands in a multivalent fashion onto magnetofluorescent nanoparticles. Our results show that (1) the synthetic nanoparticles bind to apoptotic cells, (2) there is excellent correlation with annexin V staining by microscopy, and (3) FACS analysis with nanoparticles allows the measurement of therapeutic apoptosis induction. The described nanomaterials should be useful for a variety of biomedical applications including in vivo imaging of apoptosis. IS - 3 JF - Nano Lett. AD - Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129 EP - 490 TI - A Fluorescent Nanosensor for Apoptotic Cells VL - 6 SP - 488 KW - annexin-v KW - apoptosis KW - apoptotic-cells KW - ccne-grant KW - dextran-coated-aminated-clio KW - facs KW - flow-cytometry KW - fluorescence-activated-cell-sorting KW - fluorescent-microscopy KW - jurkat-t-cells KW - mit-mgh-ccne KW - necrosis AU - Quinti, L AU - Weissleder, R AU - Tung, CH PY - 2006/03/08/ UR - http://dx.doi.org/10.1021/nl0524694 ER -