Surface-Functionalized Nanoparticle Library Yields Probes for Apoptotic Cells Export

@article{citeulike:2415627, abstract = {We have developed techniques for the efficient synthesis and screening of small libraries of surface-functionalized nanoparticles for the recognition of specific types of cells. To illustrate this concept we describe the development of a nanoparticle that preferentially recognizes apoptotic Jurkat cells in a manner similar to the apoptosis-recognizing protein annexin V. The nanoparticle, which is detectable by fluorescence or NMR relaxometry, was analyzed for the ability to recognize normal and apoptotic cells by fluorescence-activated cell sorting (FACS) analysis and fluorescence microscopy. The capability to develop nanoparticles which interact with specific target cells could be applied to the design of materials for diverse applications including quantum dots, which serve as fluorescence tracers, colloidal gold, which serves as a tracer for electron micrographs, or the crystalline forms of drugs.}, address = {Harvard Medical School, Center for Molecular Imaging Research, Building 149, 13th Street, 5403, Charlestown, MA 02129, USA, Fax: (+1) 617-726-5708}, author = {Schellenberger, Eyk A. and Reynolds, Fred and Weissleder, Ralph and Josephson, Lee}, citeulike-article-id = {2415627}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/cbic.200300713}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/107630191/ABSTRACT}, doi = {10.1002/cbic.200300713}, journal = {ChemBioChem}, keywords = {annexin-v, apoptosis, apoptotic-cells, ccne-grant, dextran-coated-aminated-clio, facs, flow-cytometry, fluorescence-activated-cell-sorting, fluorescent-microscopy, jurkat-t-cells, mit-mgh-ccne}, number = {3}, pages = {275--279}, posted-at = {2008-02-22 20:48:54}, priority = {0}, title = {Surface-Functionalized Nanoparticle Library Yields Probes for Apoptotic Cells}, url = {http://dx.doi.org/10.1002/cbic.200300713}, volume = {5}, year = {2004} }

TY - JOUR ID - citeulike:2415627 L3 - citeulike-article-id:2415627 N2 - We have developed techniques for the efficient synthesis and screening of small libraries of surface-functionalized nanoparticles for the recognition of specific types of cells. To illustrate this concept we describe the development of a nanoparticle that preferentially recognizes apoptotic Jurkat cells in a manner similar to the apoptosis-recognizing protein annexin V. The nanoparticle, which is detectable by fluorescence or NMR relaxometry, was analyzed for the ability to recognize normal and apoptotic cells by fluorescence-activated cell sorting (FACS) analysis and fluorescence microscopy. The capability to develop nanoparticles which interact with specific target cells could be applied to the design of materials for diverse applications including quantum dots, which serve as fluorescence tracers, colloidal gold, which serves as a tracer for electron micrographs, or the crystalline forms of drugs. IS - 3 JF - ChemBioChem AD - Harvard Medical School, Center for Molecular Imaging Research, Building 149, 13th Street, 5403, Charlestown, MA 02129, USA, Fax: (+1) 617-726-5708 EP - 279 TI - Surface-Functionalized Nanoparticle Library Yields Probes for Apoptotic Cells VL - 5 SP - 275 KW - annexin-v KW - apoptosis KW - apoptotic-cells KW - ccne-grant KW - dextran-coated-aminated-clio KW - facs KW - flow-cytometry KW - fluorescence-activated-cell-sorting KW - fluorescent-microscopy KW - jurkat-t-cells KW - mit-mgh-ccne AU - Schellenberger, Eyk AU - Reynolds, Fred AU - Weissleder, Ralph AU - Josephson, Lee PY - 2004/// UR - http://dx.doi.org/10.1002/cbic.200300713 ER -