Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells Export

@article{citeulike:6061299, abstract = {As the applications of industrial nanoparticles are being developed, the concerns on the environmental health are increasing. Cytotoxicities of titanium dioxide nanoparticles of different concentrations (5, 10, 20 and 40 μg/ml) were evaluated in this study using a cultured human bronchial epithelial cell line, BEAS-2B. Exposure of the cultured cells to nanoparticles led to cell death, reactive oxygen species (ROS) increase, reduced glutathione (GSH) decrease, and the induction of oxidative stress-related genes such as heme oxygenase-1, thioredoxin reductase, glutathione- S -transferase, catalase, and a hypoxia inducible gene. The ROS increase by titanium dioxide nanoparticles triggered the activation of cytosolic caspase-3 and chromatin condensation, which means that titanium dioxide nanoparticles exert cytotoxicity by an apoptotic process. Furthermore, the expressions of inflammation-related genes such as interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), TNF-a, and C-X-C motif ligand 2 (CXCL2) were also elevated. The induction of IL-8 by titanium dioxide nanoparticles was inhibited by the pre-treatment with SB203580 and PD98059, which means that the IL-8 was induced through p38 mitogen-acitvated protein kinase (MAPK) pathway and/or extracellular signal (ERK) pathway. Uptake of the nanoparticles into the cultured cells was observed and titanium dioxide nanoparticles seemed to penetrate into the cytoplasm and locate in the peri-region of the nucleus as aggregated particles, which may induce direct interactions between the particles and cellular molecules, to cause adverse biological responses.}, author = {Park, Eun-Jung and Yi, Jongheop and Chung, Kyu-Hyuck and Ryu, Doug-Young and Choi, Jinhee and Park, Kwangsik}, citeulike-article-id = {6061299}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.toxlet.2008.06.869}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0378427408010552}, day = {28}, doi = {10.1016/j.toxlet.2008.06.869}, issn = {03784274}, journal = {Toxicology Letters}, keywords = {cell-viability, metal-oxide, reactive-oxygen-species, toxicity}, month = {August}, number = {3}, pages = {222--229}, posted-at = {2009-11-03 18:44:14}, priority = {2}, title = {Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells}, url = {http://dx.doi.org/10.1016/j.toxlet.2008.06.869}, volume = {180}, year = {2008} }

TY - JOUR ID - citeulike:6061299 L3 - citeulike-article-id:6061299 N2 - As the applications of industrial nanoparticles are being developed, the concerns on the environmental health are increasing. Cytotoxicities of titanium dioxide nanoparticles of different concentrations (5, 10, 20 and 40 μg/ml) were evaluated in this study using a cultured human bronchial epithelial cell line, BEAS-2B. Exposure of the cultured cells to nanoparticles led to cell death, reactive oxygen species (ROS) increase, reduced glutathione (GSH) decrease, and the induction of oxidative stress-related genes such as heme oxygenase-1, thioredoxin reductase, glutathione- S -transferase, catalase, and a hypoxia inducible gene. The ROS increase by titanium dioxide nanoparticles triggered the activation of cytosolic caspase-3 and chromatin condensation, which means that titanium dioxide nanoparticles exert cytotoxicity by an apoptotic process. Furthermore, the expressions of inflammation-related genes such as interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), TNF-a, and C-X-C motif ligand 2 (CXCL2) were also elevated. The induction of IL-8 by titanium dioxide nanoparticles was inhibited by the pre-treatment with SB203580 and PD98059, which means that the IL-8 was induced through p38 mitogen-acitvated protein kinase (MAPK) pathway and/or extracellular signal (ERK) pathway. Uptake of the nanoparticles into the cultured cells was observed and titanium dioxide nanoparticles seemed to penetrate into the cytoplasm and locate in the peri-region of the nucleus as aggregated particles, which may induce direct interactions between the particles and cellular molecules, to cause adverse biological responses. IS - 3 JF - Toxicology Letters SN - 03784274 EP - 229 TI - Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells VL - 180 SP - 222 KW - cell-viability KW - metal-oxide KW - reactive-oxygen-species KW - toxicity AU - Park, Eun-Jung AU - Yi, Jongheop AU - Chung, Kyu-Hyuck AU - Ryu, Doug-Young AU - Choi, Jinhee AU - Park, Kwangsik PY - 2008/08/28/ UR - http://dx.doi.org/10.1016/j.toxlet.2008.06.869 ER -