Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation. Export

@article{citeulike:2624777, abstract = {Laminin-1 is essential for early embryonic basement membrane assembly and differentiation. Several steps can be distinguished, i.e., the expression of laminin and companion matrix components, their accumulation on the cell surface and assembly into basement membrane between endoderm and inner cell mass, and the ensuing differentiation of epiblast. In this study, we used differentiating embryoid bodies derived from mouse embryonic stem cells null for gamma1-laminin, beta1-integrin and alpha/beta-dystroglycan to dissect the contributions of laminin domains and interacting receptors to this process. We found that (a) laminin enables beta1-integrin-null embryoid bodies to assemble basement membrane and achieve epiblast with beta1-integrin enabling expression of the laminin alpha1 subunit; (b) basement membrane assembly and differentiation require laminin polymerization in conjunction with cell anchorage, the latter critically dependent upon a heparin-binding locus within LG module-4; (c) dystroglycan is not uniquely required for basement membrane assembly or initial differentiation; (d) dystroglycan and integrin cooperate to sustain survival of the epiblast and regulate laminin expression; and (e) laminin, acting via beta1-integrin through LG1-3 and requiring polymerization, can regulate dystroglycan expression.}, address = {Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey (UMDNJ), Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.}, author = {Li, S. and Harrison, D. and Carbonetto, S. and Fassler, R. and Smyth, N. and Edgar, D. and Yurchenco, P. D.}, citeulike-article-id = {2624777}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200203073}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12082085}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12082085}, day = {24}, doi = {10.1083/jcb.200203073}, issn = {0021-9525}, journal = {The Journal of cell biology}, keywords = {dag1}, month = {June}, number = {7}, pages = {1279--1290}, posted-at = {2008-04-03 02:49:46}, priority = {2}, title = {Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation.}, url = {http://dx.doi.org/10.1083/jcb.200203073}, volume = {157}, year = {2002} }

TY - JOUR ID - citeulike:2624777 L3 - citeulike-article-id:2624777 N2 - Laminin-1 is essential for early embryonic basement membrane assembly and differentiation. Several steps can be distinguished, i.e., the expression of laminin and companion matrix components, their accumulation on the cell surface and assembly into basement membrane between endoderm and inner cell mass, and the ensuing differentiation of epiblast. In this study, we used differentiating embryoid bodies derived from mouse embryonic stem cells null for gamma1-laminin, beta1-integrin and alpha/beta-dystroglycan to dissect the contributions of laminin domains and interacting receptors to this process. We found that (a) laminin enables beta1-integrin-null embryoid bodies to assemble basement membrane and achieve epiblast with beta1-integrin enabling expression of the laminin alpha1 subunit; (b) basement membrane assembly and differentiation require laminin polymerization in conjunction with cell anchorage, the latter critically dependent upon a heparin-binding locus within LG module-4; (c) dystroglycan is not uniquely required for basement membrane assembly or initial differentiation; (d) dystroglycan and integrin cooperate to sustain survival of the epiblast and regulate laminin expression; and (e) laminin, acting via beta1-integrin through LG1-3 and requiring polymerization, can regulate dystroglycan expression. IS - 7 JF - The Journal of cell biology SN - 0021-9525 AD - Department of Pathology and Laboratory Medicine, University of Medicine and Dentistry of New Jersey (UMDNJ), Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA. EP - 1290 TI - Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation. VL - 157 SP - 1279 KW - dag1 AU - Li, S AU - Harrison, D AU - Carbonetto, S AU - Fassler, R AU - Smyth, N AU - Edgar, D AU - Yurchenco, PD PY - 2002/06/24/ UR - http://dx.doi.org/10.1083/jcb.200203073 ER -