Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Export

@article{citeulike:3511201, abstract = {Stem cell lines that provide a renewable and scaleable supply of central nervous system cell types would constitute an invaluable resource for basic and applied neurobiology. Here we describe the generation and long-term expansion of multiple human foetal neural stem (NS) cell lines in monolayer culture without genetic immortalization. Adherent human NS cells are propagated in the presence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), under which conditions they stably express neural precursor markers and exhibit negligible differentiation into neurons or glia. However, they produce astrocytes, oligodendrocytes, and neurons upon exposure to appropriate differentiation factors. Single cell cloning demonstrates that human NS cells are tripotent. They retain a diploid karyotype and constant neurogenic capacity after over 100 generations. In contrast to human neurospheres, we observe no requirement for the cytokine leukaemia inhibitory factor (LIF) for continued expansion of adherent human NS cells. Human NS cells can be stably transfected to provide reporter lines and readily imaged in live monolayer cultures, creating the potential for high content genetic and chemical screens.}, author = {Sun, Y. and Pollard, S. and Conti, L. and Toselli, M. and Biella, G. and Parkin, G. and Willatt, L. and Falk, A. and Cattaneo, E. and Smith, A.}, citeulike-article-id = {3511201}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.mcn.2008.02.014}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18450476}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18450476}, doi = {10.1016/j.mcn.2008.02.014}, issn = {1095-9327}, journal = {Molecular and cellular neurosciences}, keywords = {npc, psancam}, month = {June}, number = {2}, pages = {245--258}, posted-at = {2008-11-14 05:22:59}, priority = {5}, title = {Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture.}, url = {http://dx.doi.org/10.1016/j.mcn.2008.02.014}, volume = {38}, year = {2008} }

TY - JOUR ID - citeulike:3511201 L3 - citeulike-article-id:3511201 N2 - Stem cell lines that provide a renewable and scaleable supply of central nervous system cell types would constitute an invaluable resource for basic and applied neurobiology. Here we describe the generation and long-term expansion of multiple human foetal neural stem (NS) cell lines in monolayer culture without genetic immortalization. Adherent human NS cells are propagated in the presence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), under which conditions they stably express neural precursor markers and exhibit negligible differentiation into neurons or glia. However, they produce astrocytes, oligodendrocytes, and neurons upon exposure to appropriate differentiation factors. Single cell cloning demonstrates that human NS cells are tripotent. They retain a diploid karyotype and constant neurogenic capacity after over 100 generations. In contrast to human neurospheres, we observe no requirement for the cytokine leukaemia inhibitory factor (LIF) for continued expansion of adherent human NS cells. Human NS cells can be stably transfected to provide reporter lines and readily imaged in live monolayer cultures, creating the potential for high content genetic and chemical screens. IS - 2 JF - Molecular and cellular neurosciences SN - 1095-9327 EP - 258 TI - Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. VL - 38 SP - 245 KW - npc KW - psancam AU - Sun, Y AU - Pollard, S AU - Conti, L AU - Toselli, M AU - Biella, G AU - Parkin, G AU - Willatt, L AU - Falk, A AU - Cattaneo, E AU - Smith, A PY - 2008/06// UR - http://dx.doi.org/10.1016/j.mcn.2008.02.014 ER -