Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus. Export

@article{citeulike:3511305, abstract = {Synapsin III is a synaptic vesicle-associated protein that is expressed in cells of the subgranular layer of the hippocampal dentate gyrus, a brain region known to sustain substantial levels of neurogenesis into adulthood. Here we tested the hypothesis that synapsin III plays a role in adult neurogenesis with synapsin III knockout and wild-type mice. Immunocytochemistry of the adult hippocampal dentate gyrus revealed that synapsin III colocalizes with markers of neural progenitor cell development (nestin, PSA-NCAM, NeuN, and Tuj1) but did not colocalize with markers of mitosis (Ki67 and PCNA). Because neurogenesis consists of a number of stages, the proliferation, survival, and differentiation of neural progenitor cells were systematically quantitated in the hippocampal dentate gyrus of adult synapsin III knockout and wild-type mice. We found a 30\% decrease in proliferation and a 55\% increase in survival of neural progenitor cells in synapsin III knockout mice. We also observed a 6\% increase in the number of neural progenitor cells that differentiated into neurons. No difference in the volume of the dentate gyrus was observed between synapsin III knockout and wild-type mice. Collectively, our results demonstrate a novel role for synapsin III in regulating the proliferation of neural progenitor cells in the adult hippocampal dentate gyrus. These findings suggest a distinct function for this synaptic vesicle protein, in addition to its role in neurotransmission.}, author = {Kao, H. T. and Li, P. and Chao, H. M. and Janoschka, S. and Pham, K. and Feng, J. and Mcewen, B. S. and Greengard, P. and Pieribone, V. A. and Porton, B.}, citeulike-article-id = {3511305}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/cne.21643}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18271024}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18271024}, day = {20}, doi = {10.1002/cne.21643}, issn = {1096-9861}, journal = {The Journal of comparative neurology}, keywords = {npc, psancam}, month = {April}, number = {6}, pages = {1860--1870}, posted-at = {2008-11-14 05:51:41}, priority = {2}, title = {Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus.}, url = {http://dx.doi.org/10.1002/cne.21643}, volume = {507}, year = {2008} }

TY - JOUR ID - citeulike:3511305 L3 - citeulike-article-id:3511305 N2 - Synapsin III is a synaptic vesicle-associated protein that is expressed in cells of the subgranular layer of the hippocampal dentate gyrus, a brain region known to sustain substantial levels of neurogenesis into adulthood. Here we tested the hypothesis that synapsin III plays a role in adult neurogenesis with synapsin III knockout and wild-type mice. Immunocytochemistry of the adult hippocampal dentate gyrus revealed that synapsin III colocalizes with markers of neural progenitor cell development (nestin, PSA-NCAM, NeuN, and Tuj1) but did not colocalize with markers of mitosis (Ki67 and PCNA). Because neurogenesis consists of a number of stages, the proliferation, survival, and differentiation of neural progenitor cells were systematically quantitated in the hippocampal dentate gyrus of adult synapsin III knockout and wild-type mice. We found a 30% decrease in proliferation and a 55% increase in survival of neural progenitor cells in synapsin III knockout mice. We also observed a 6% increase in the number of neural progenitor cells that differentiated into neurons. No difference in the volume of the dentate gyrus was observed between synapsin III knockout and wild-type mice. Collectively, our results demonstrate a novel role for synapsin III in regulating the proliferation of neural progenitor cells in the adult hippocampal dentate gyrus. These findings suggest a distinct function for this synaptic vesicle protein, in addition to its role in neurotransmission. IS - 6 JF - The Journal of comparative neurology SN - 1096-9861 EP - 1870 TI - Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus. VL - 507 SP - 1860 KW - npc KW - psancam AU - Kao, HT AU - Li, P AU - Chao, HM AU - Janoschka, S AU - Pham, K AU - Feng, J AU - Mcewen, BS AU - Greengard, P AU - Pieribone, VA AU - Porton, B PY - 2008/04/20/ UR - http://dx.doi.org/10.1002/cne.21643 ER -