Generation of reactive oxygen species from porous silicon microparticles in cell culture medium Export

@article{citeulike:6055824, abstract = {Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drug-delivery vehicle. Here, we tested the biocompatibility of non-treated and thermally-oxidized porous silicon particles using an indirect cell viability assay. Initial direct cell culture on porous silicon determined that human lens epithelial cells only poorly adhered to non-treated porous silicon. Using an indirect cell culture assay, we found that non-treated microparticles caused complete cell death, indicating that these particles generated a toxic product in cell culture medium. In contrast, thermally-oxidized microparticles did not reduce cell viability significantly. We found evidence for the generation of reactive oxygen species (ROS) by means of the fluorescent probe 2prime,7prime-dichlorofluorescin. Our results suggest that non-treated porous silicon microparticles produced ROS, which interacted with the components of the cell culture medium, leading to the formation of cytotoxic species. Oxidation of porous silicon microparticles not only mitigated, but also abolished the toxic effects. {\copyright} 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009}, address = {Faculty of Science and Engineering, School of Chemistry, Physics and Earth Sciences, Flinders University, Adelaide, South Australia; Department of Ophthalmology, Flinders University, Adelaide, South Australia; pSiMedica, Malvern, Worcestershire, United Kingdom}, author = {Low, Suet P. and Williams, Keryn A. and Canham, Leigh T. and Voelcker, Nicolas H.}, citeulike-article-id = {6055824}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/jbm.a.32610}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122603101/ABSTRACT}, doi = {10.1002/jbm.a.32610}, issn = {1552-4965}, journal = {Journal of Biomedical Materials Research Part A}, keywords = {microparticles, porous\_silicon, toxicity}, number = {9999}, pages = {NA+}, posted-at = {2009-11-02 10:30:22}, priority = {2}, title = {Generation of reactive oxygen species from porous silicon microparticles in cell culture medium}, url = {http://dx.doi.org/10.1002/jbm.a.32610}, volume = {9999}, year = {2009} }

TY - JOUR ID - citeulike:6055824 L3 - citeulike-article-id:6055824 N2 - Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drug-delivery vehicle. Here, we tested the biocompatibility of non-treated and thermally-oxidized porous silicon particles using an indirect cell viability assay. Initial direct cell culture on porous silicon determined that human lens epithelial cells only poorly adhered to non-treated porous silicon. Using an indirect cell culture assay, we found that non-treated microparticles caused complete cell death, indicating that these particles generated a toxic product in cell culture medium. In contrast, thermally-oxidized microparticles did not reduce cell viability significantly. We found evidence for the generation of reactive oxygen species (ROS) by means of the fluorescent probe 2prime,7prime-dichlorofluorescin. Our results suggest that non-treated porous silicon microparticles produced ROS, which interacted with the components of the cell culture medium, leading to the formation of cytotoxic species. Oxidation of porous silicon microparticles not only mitigated, but also abolished the toxic effects. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009 IS - 9999 JF - Journal of Biomedical Materials Research Part A SN - 1552-4965 AD - Faculty of Science and Engineering, School of Chemistry, Physics and Earth Sciences, Flinders University, Adelaide, South Australia; Department of Ophthalmology, Flinders University, Adelaide, South Australia; pSiMedica, Malvern, Worcestershire, United Kingdom TI - Generation of reactive oxygen species from porous silicon microparticles in cell culture medium VL - 9999 SP - NA KW - microparticles KW - porous_silicon KW - toxicity AU - Low, Suet AU - Williams, Keryn AU - Canham, Leigh AU - Voelcker, Nicolas PY - 2009/// UR - http://dx.doi.org/10.1002/jbm.a.32610 ER -