Architecture-Dependent Noise Discriminates Functionally Analogous Differentiation Circuits Export

@article{citeulike:5989171, abstract = {Gene regulatory circuits with different architectures (patterns of regulatory interactions) can generate similar dynamics. This raises the question of why a particular circuit architecture is selected to implement a given cellular process. To investigate this problem, we compared the Bacillus subtilis circuit that regulates differentiation into the competence state to an engineered circuit with an alternative architecture (SynEx) in silico and in vivo. Time-lapse microscopy measurements showed that SynEx cells generated competence dynamics similar to native cells and reconstituted the physiology of differentiation. However, architectural differences between the circuits altered the dynamic distribution of stochastic fluctuations (noise) during circuit operation. This distinction in noise causes functional differences between the circuits by selectively controlling the timing of competence episodes and response of the system to various DNA concentrations. These results reveal a tradeoff between temporal precision and physiological response range that is controlled by distinct noise characteristics of alternative circuit architectures.}, author = {\c{C}aatay, Tolga and Turcotte, Marc and Elowitz, Michael B. and Garcia-Ojalvo, Jordi and S\"{u}el, G\"{u}rol M.}, citeulike-article-id = {5989171}, citeulike-linkout-0 = {http://www.cell.com//abstract/S0092-8674(09)01033-2}, day = {30}, keywords = {circuit\_architectures, fluctuations, functional\_differences, network, noise, phenotype, robustness, single\_cell, time\_lapse}, month = {October}, number = {3}, pages = {512--522}, posted-at = {2009-11-04 05:16:50}, priority = {5}, title = {Architecture-Dependent Noise Discriminates Functionally Analogous Differentiation Circuits}, url = {http://www.cell.com//abstract/S0092-8674(09)01033-2}, volume = {139}, year = {2009} }

TY - JOUR ID - citeulike:5989171 L3 - citeulike-article-id:5989171 N2 - Gene regulatory circuits with different architectures (patterns of regulatory interactions) can generate similar dynamics. This raises the question of why a particular circuit architecture is selected to implement a given cellular process. To investigate this problem, we compared the Bacillus subtilis circuit that regulates differentiation into the competence state to an engineered circuit with an alternative architecture (SynEx) in silico and in vivo. Time-lapse microscopy measurements showed that SynEx cells generated competence dynamics similar to native cells and reconstituted the physiology of differentiation. However, architectural differences between the circuits altered the dynamic distribution of stochastic fluctuations (noise) during circuit operation. This distinction in noise causes functional differences between the circuits by selectively controlling the timing of competence episodes and response of the system to various DNA concentrations. These results reveal a tradeoff between temporal precision and physiological response range that is controlled by distinct noise characteristics of alternative circuit architectures. IS - 3 EP - 522 TI - Architecture-Dependent Noise Discriminates Functionally Analogous Differentiation Circuits VL - 139 SP - 512 KW - circuit_architectures KW - fluctuations KW - functional_differences KW - network KW - noise KW - phenotype KW - robustness KW - single_cell KW - time_lapse AU - Çaatay, Tolga AU - Turcotte, Marc AU - Elowitz, Michael AU - Garcia-Ojalvo, Jordi AU - Süel, Gürol PY - 2009/10/30/ UR - http://www.cell.com//abstract/S0092-8674(09)01033-2 ER -