High potency silencing by single-stranded boranophosphate siRNA. Export

@article{citeulike:1206957, abstract = {In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents.}, address = {Department of Molecular Genetics and Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA.}, author = {Hall, A. H. and Wan, J. and Spesock, A. and Sergueeva, Z. and Shaw, B. R. and Alexander, K. A.}, citeulike-article-id = {1206957}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/16717282}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=16717282}, issn = {1362-4962}, journal = {Nucleic Acids Res}, keywords = {boranophosphate, sirna}, number = {9}, pages = {2773--2781}, posted-at = {2007-04-05 04:14:18}, priority = {2}, title = {High potency silencing by single-stranded boranophosphate siRNA.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16717282}, volume = {34}, year = {2006} }

TY - JOUR ID - citeulike:1206957 L3 - citeulike-article-id:1206957 N2 - In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents. IS - 9 JF - Nucleic Acids Res SN - 1362-4962 AD - Department of Molecular Genetics and Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USA. EP - 2781 TI - High potency silencing by single-stranded boranophosphate siRNA. VL - 34 SP - 2773 KW - boranophosphate KW - sirna AU - Hall, AH AU - Wan, J AU - Spesock, A AU - Sergueeva, Z AU - Shaw, BR AU - Alexander, KA PY - 2006/// UR - http://view.ncbi.nlm.nih.gov/pubmed/16717282 ER -