MicroRNA fingerprints during human megakaryocytopoiesis. Export

@article{citeulike:561162, abstract = {microRNAs are a highly conserved class of noncoding RNAs with important regulatory functions in proliferation, apoptosis, development, and differentiation. To discover novel regulatory pathways during megakaryocytic differentiation, we performed microRNA expression profiling of in vitro-differentiated megakaryocytes derived from CD34(+) hematopoietic progenitors. The main finding was down-regulation of miR-10a, miR-126, miR-106, miR-10b, miR-17 and miR-20. Hypothetically, the down-regulation of microRNAs unblocks target genes involved in differentiation. We confirmed in vitro and in vivo that miR-130a targets the transcription factor MAFB, which is involved in the activation of the GPIIB promoter, a key protein for platelet physiology. In addition, we found that miR-10a expression in differentiated megakaryocytes is inverse to that of HOXA1, and we showed that HOXA1 is a direct target of miR-10a. Finally, we compared the microRNA expression of megakaryoblastic leukemic cell lines with that of in vitro differentiated megakaryocytes and CD34(+) progenitors. This analysis revealed up-regulation of miR-101, miR-126, miR-99a, miR-135, and miR-20. Our data delineate the expression of microRNAs during megakaryocytopoiesis and suggest a regulatory role of microRNAs in this process by targeting megakaryocytic transcription factors.}, address = {Departments of Molecular Virology, Immunology, and Human Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210;}, author = {Garzon, Ramiro and Pichiorri, Flavia and Palumbo, Tiziana and Iuliano, Rodolfo and Cimmino, Amelia and Aqeilan, Rami and Volinia, Stefano and Bhatt, Darshna and Alder, Hansjuerg and Marcucci, Guido and Calin, George A A. and Liu, Chang-Gong G. and Bloomfield, Clara D D. and Andreeff, Michael and Croce, Carlo M M.}, citeulike-article-id = {561162}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0600587103}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16549775}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16549775}, day = {20}, doi = {10.1073/pnas.0600587103}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {function, microrna, ncrna}, month = {March}, posted-at = {2006-07-13 07:51:47}, priority = {4}, title = {MicroRNA fingerprints during human megakaryocytopoiesis.}, url = {http://dx.doi.org/10.1073/pnas.0600587103}, year = {2006} }

TY - JOUR ID - citeulike:561162 L3 - citeulike-article-id:561162 N2 - microRNAs are a highly conserved class of noncoding RNAs with important regulatory functions in proliferation, apoptosis, development, and differentiation. To discover novel regulatory pathways during megakaryocytic differentiation, we performed microRNA expression profiling of in vitro-differentiated megakaryocytes derived from CD34(+) hematopoietic progenitors. The main finding was down-regulation of miR-10a, miR-126, miR-106, miR-10b, miR-17 and miR-20. Hypothetically, the down-regulation of microRNAs unblocks target genes involved in differentiation. We confirmed in vitro and in vivo that miR-130a targets the transcription factor MAFB, which is involved in the activation of the GPIIB promoter, a key protein for platelet physiology. In addition, we found that miR-10a expression in differentiated megakaryocytes is inverse to that of HOXA1, and we showed that HOXA1 is a direct target of miR-10a. Finally, we compared the microRNA expression of megakaryoblastic leukemic cell lines with that of in vitro differentiated megakaryocytes and CD34(+) progenitors. This analysis revealed up-regulation of miR-101, miR-126, miR-99a, miR-135, and miR-20. Our data delineate the expression of microRNAs during megakaryocytopoiesis and suggest a regulatory role of microRNAs in this process by targeting megakaryocytic transcription factors. JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Departments of Molecular Virology, Immunology, and Human Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210; TI - MicroRNA fingerprints during human megakaryocytopoiesis. KW - function KW - microrna KW - ncrna AU - Garzon, Ramiro AU - Pichiorri, Flavia AU - Palumbo, Tiziana AU - Iuliano, Rodolfo AU - Cimmino, Amelia AU - Aqeilan, Rami AU - Volinia, Stefano AU - Bhatt, Darshna AU - Alder, Hansjuerg AU - Marcucci, Guido AU - Calin, George A AU - Liu, Chang-Gong AU - Bloomfield, Clara D AU - Andreeff, Michael AU - Croce, Carlo M PY - 2006/03/20/ UR - http://dx.doi.org/10.1073/pnas.0600587103 ER -