Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes. Export

@article{citeulike:742083, abstract = {In Drosophila melanogaster, the Dicer-2/R2D2 complex initiates RNA interference (RNAi) by processing long double-stranded RNA (dsRNA) into small interfering RNA (siRNA). Recent biochemical studies suggest that the Dcr-2/R2D2 complex also facilitates incorporation of siRNA into the RNA-induced silencing complex (siRISC). Here we present genetic evidence that R2D2 and Dcr-2 are both required for loading siRNA onto the siRISC complex. Consistent with this, only the Dcr-2/R2D2 complex, but neither Dcr-2 nor R2D2 alone, can efficiently interact with duplex siRNA. Furthermore, both dsRNA-binding domains of R2D2 are critical for binding to siRNA and promoting assembly of the siRISC complexes.}, address = {1Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.}, author = {Liu, Xiang and Jiang, Feng and Kalidas, Savitha and Smith, Dean and Liu, Qinghua}, citeulike-article-id = {742083}, citeulike-linkout-0 = {http://dx.doi.org/10.1261/rna.101606}, citeulike-linkout-1 = {http://www.rnajournal.org/cgi/content/abstract/12/8/1514}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16775303}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16775303}, day = {14}, doi = {10.1261/rna.101606}, issn = {1355-8382}, journal = {RNA}, keywords = {dicer, risc, rnai, sirna}, month = {June}, posted-at = {2006-08-18 02:53:12}, priority = {2}, title = {Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes.}, url = {http://dx.doi.org/10.1261/rna.101606}, year = {2006} }

TY - JOUR ID - citeulike:742083 L3 - citeulike-article-id:742083 N2 - In Drosophila melanogaster, the Dicer-2/R2D2 complex initiates RNA interference (RNAi) by processing long double-stranded RNA (dsRNA) into small interfering RNA (siRNA). Recent biochemical studies suggest that the Dcr-2/R2D2 complex also facilitates incorporation of siRNA into the RNA-induced silencing complex (siRISC). Here we present genetic evidence that R2D2 and Dcr-2 are both required for loading siRNA onto the siRISC complex. Consistent with this, only the Dcr-2/R2D2 complex, but neither Dcr-2 nor R2D2 alone, can efficiently interact with duplex siRNA. Furthermore, both dsRNA-binding domains of R2D2 are critical for binding to siRNA and promoting assembly of the siRISC complexes. JF - RNA SN - 1355-8382 AD - 1Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA. TI - Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes. KW - dicer KW - risc KW - rnai KW - sirna AU - Liu, Xiang AU - Jiang, Feng AU - Kalidas, Savitha AU - Smith, Dean AU - Liu, Qinghua PY - 2006/06/14/ UR - http://dx.doi.org/10.1261/rna.101606 ER -