Identification of heterotic metabolite QTL in <i>Arabidopsis thaliana</i> RIL and IL populations Export

@article{citeulike:5679541, abstract = {Two mapping populations of a cross between the Arabidopsis thaliana accessions Col-0 and C24 were cultivated and analyzed with respect to the levels of 181 metabolites to elucidate the biological phenomenon of heterosis at the metabolic level. The relative mid-parent heterosis in the F1 hybrids was \&lt;20\% for most metabolic traits. The first mapping population consisting of 369 recombinant inbred lines (RILs) and their test cross progeny with both parents allowed us to determine the position and effect of 147 quantitative trait loci (QTL) for metabolite absolute mid-parent heterosis (aMPH). Furthermore, we identified 153 and 83 QTL for augmented additive (Z1) and dominance effects (Z2), respectively. We identified putative candidate genes for these QTL using the aracyc database (http://www.arabidopsis.org/biocyc), and calculated the average degree of dominance, which was within the dominance and over-dominance range for most metabolites. Analyzing a second population of 41 introgression lines (ILs) and their test crosses with the recurrent parent, we identified 634 significant differences in metabolite levels. Nine per cent of these effects were classified as over-dominant, according to the mode of inheritance. A comparison of both approaches suggested epistasis as a major contributor to metabolite heterosis in Arabidopsis. A linear combination of metabolite levels was shown to significantly correlate with biomass heterosis (r = 0.62).}, address = {Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany; Department of Bioinformatics, University of Potsdam, 14476 Potsdam-Golm, Germany; Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany; Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70599 Stuttgart, Germany}, author = {Lisec, Jan and Steinfath, Matthias and Meyer, Rhonda C. and Selbig, Joachim and Melchinger, Albrecht E. and Willmitzer, Lothar and Altmann, Thomas}, citeulike-article-id = {5679541}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-313X.2009.03910.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/tpj/2009/00000059/00000005/art00008}, citeulike-linkout-2 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122372937/ABSTRACT}, doi = {10.1111/j.1365-313X.2009.03910.x}, issn = {1365-313X}, journal = {The Plant Journal}, keywords = {epistasis, heterosis, qtl, ril}, number = {5}, pages = {777--788}, posted-at = {2009-09-28 12:17:34}, priority = {2}, publisher = {Blackwell Publishing}, title = {Identification of heterotic metabolite QTL in <i>Arabidopsis thaliana</i> RIL and IL populations}, url = {http://dx.doi.org/10.1111/j.1365-313X.2009.03910.x}, volume = {59}, year = {2009} }

TY - JOUR ID - citeulike:5679541 L3 - citeulike-article-id:5679541 N2 - Two mapping populations of a cross between the Arabidopsis thaliana accessions Col-0 and C24 were cultivated and analyzed with respect to the levels of 181 metabolites to elucidate the biological phenomenon of heterosis at the metabolic level. The relative mid-parent heterosis in the F1 hybrids was &lt;20% for most metabolic traits. The first mapping population consisting of 369 recombinant inbred lines (RILs) and their test cross progeny with both parents allowed us to determine the position and effect of 147 quantitative trait loci (QTL) for metabolite absolute mid-parent heterosis (aMPH). Furthermore, we identified 153 and 83 QTL for augmented additive (Z1) and dominance effects (Z2), respectively. We identified putative candidate genes for these QTL using the aracyc database (http://www.arabidopsis.org/biocyc), and calculated the average degree of dominance, which was within the dominance and over-dominance range for most metabolites. Analyzing a second population of 41 introgression lines (ILs) and their test crosses with the recurrent parent, we identified 634 significant differences in metabolite levels. Nine per cent of these effects were classified as over-dominant, according to the mode of inheritance. A comparison of both approaches suggested epistasis as a major contributor to metabolite heterosis in Arabidopsis. A linear combination of metabolite levels was shown to significantly correlate with biomass heterosis (r = 0.62). IS - 5 JF - The Plant Journal SN - 1365-313X AD - Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany; Department of Bioinformatics, University of Potsdam, 14476 Potsdam-Golm, Germany; Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany; Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70599 Stuttgart, Germany EP - 788 TI - Identification of heterotic metabolite QTL in <i>Arabidopsis thaliana</i> RIL and IL populations PB - Blackwell Publishing VL - 59 SP - 777 KW - epistasis KW - heterosis KW - qtl KW - ril AU - Lisec, Jan AU - Steinfath, Matthias AU - Meyer, Rhonda AU - Selbig, Joachim AU - Melchinger, Albrecht AU - Willmitzer, Lothar AU - Altmann, Thomas PY - 2009/// UR - http://dx.doi.org/10.1111/j.1365-313X.2009.03910.x ER -