Expression of the Arabidopsis mutant abi1 gene alters ABA sensitivity, stomatal development and growth morphology in Grey poplars (Populus x canescens (Ait.) Sm.). Export

@article{citeulike:5974940, abstract = {The consequences of altered ABA sensitivity in grey poplar development (Populus x canescens (Ait.) Sm.) were examined by ectopic expression of Arabidopsis mutant abi1 gene. The expression resulted in an ABA-insensitive phenotype revealed by a strong tendency of abi1 poplars to wilt, impaired responsiveness of their stomata to ABA, and an ABA-resistant bud outgrowth. These plants therefore required cultivation under very humid conditions to prevent drought stress symptoms. Morphological alterations became evident when comparing abi1 poplars with poplars expressing Arabidopsis non-mutant ABI1 or wild type (wt) plants. abi1 poplars showed increased stomatal size, enhanced shoot growth and retarded leaf and root development. The increased stomatal size and its reversion to the size of wt plants by exogenous ABA indicate a role for ABA in regulating stomatal development. Enhanced shoot growth and retarded leaf and root development support the hypothesis that ABA acts independently from drought stress as a negative regulator of growth in shoots and as a positive regulator of growth in leaves and roots. In shoots, we observed an interaction of ABA with ethylene: abi1 poplars exhibited elevated ethylene production, and the ethylene perception inhibitor Ag(+) antagonised the enhanced shoot growth. Thus, we provide evidence that ABA acts as negative regulator of shoot growth in non-stressed poplars by restricting ethylene production. Furthermore, we show that ABA has a role in regulating shoot branching by inhibiting lateral bud outgrowth.}, author = {Arend, Matthias and Schnitzler, Jorg-Peter P. and Ehlting, Barbara and Hansch, Robert and Lange, Theo and Rennenberg, Heinz and Himmelbach, Axel and Grill, Erwin and Fromm, Jorg}, citeulike-article-id = {5974940}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.109.144956}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19837818}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19837818}, day = {16}, doi = {10.1104/pp.109.144956}, issn = {0032-0889}, journal = {Plant physiology}, keywords = {aba, abiotic-stress, stress}, month = {October}, posted-at = {2009-10-20 16:27:53}, priority = {2}, title = {Expression of the Arabidopsis mutant abi1 gene alters ABA sensitivity, stomatal development and growth morphology in Grey poplars (Populus x canescens (Ait.) Sm.).}, url = {http://dx.doi.org/10.1104/pp.109.144956}, year = {2009} }

TY - JOUR ID - citeulike:5974940 L3 - citeulike-article-id:5974940 N2 - The consequences of altered ABA sensitivity in grey poplar development (Populus x canescens (Ait.) Sm.) were examined by ectopic expression of Arabidopsis mutant abi1 gene. The expression resulted in an ABA-insensitive phenotype revealed by a strong tendency of abi1 poplars to wilt, impaired responsiveness of their stomata to ABA, and an ABA-resistant bud outgrowth. These plants therefore required cultivation under very humid conditions to prevent drought stress symptoms. Morphological alterations became evident when comparing abi1 poplars with poplars expressing Arabidopsis non-mutant ABI1 or wild type (wt) plants. abi1 poplars showed increased stomatal size, enhanced shoot growth and retarded leaf and root development. The increased stomatal size and its reversion to the size of wt plants by exogenous ABA indicate a role for ABA in regulating stomatal development. Enhanced shoot growth and retarded leaf and root development support the hypothesis that ABA acts independently from drought stress as a negative regulator of growth in shoots and as a positive regulator of growth in leaves and roots. In shoots, we observed an interaction of ABA with ethylene: abi1 poplars exhibited elevated ethylene production, and the ethylene perception inhibitor Ag(+) antagonised the enhanced shoot growth. Thus, we provide evidence that ABA acts as negative regulator of shoot growth in non-stressed poplars by restricting ethylene production. Furthermore, we show that ABA has a role in regulating shoot branching by inhibiting lateral bud outgrowth. JF - Plant physiology SN - 0032-0889 TI - Expression of the Arabidopsis mutant abi1 gene alters ABA sensitivity, stomatal development and growth morphology in Grey poplars (Populus x canescens (Ait.) Sm.). KW - aba KW - abiotic-stress KW - stress AU - Arend, Matthias AU - Schnitzler, Jorg-Peter AU - Ehlting, Barbara AU - Hansch, Robert AU - Lange, Theo AU - Rennenberg, Heinz AU - Himmelbach, Axel AU - Grill, Erwin AU - Fromm, Jorg PY - 2009/10/16/ UR - http://dx.doi.org/10.1104/pp.109.144956 ER -