New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening. Export

@article{citeulike:4179613, abstract = {The identification and characterization of previously unidentified signal transduction molecules has expanded our understanding of biological systems and facilitated the development of mechanism-based therapeutics. We present a highly validated small interfering RNA (siRNA) screen that functionally annotates the human genome for modulation of the Wnt/beta-catenin signal transduction pathway. Merging these functional data with an extensive Wnt/beta-catenin protein interaction network produces an integrated physical and functional map of the pathway. The power of this approach is illustrated by the positioning of siRNA screen hits into discrete physical complexes of proteins. Similarly, this approach allows one to filter discoveries made through protein-protein interaction screens for functional contribution to the phenotype of interest. Using this methodology, we characterized AGGF1 as a nuclear chromatin-associated protein that participates in beta-catenin-mediated transcription in human colon cancer cells.}, author = {Major, M. B. and Roberts, B. S. and Berndt, J. D. and Marine, S. and Anastas, J. and Chung, N. and Ferrer, M. and Yi, X. and Stoick-Cooper, C. L. and von Haller, P. D. and Kategaya, L. and Chien, A. and Angers, S. and MacCoss, M. and Cleary, M. A. and Arthur, W. T. and Moon, R. T.}, citeulike-article-id = {4179613}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/scisignal.2000037}, citeulike-linkout-1 = {http://stke.sciencemag.org/cgi/content/abstract/1/45/ra12}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19001663}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19001663}, doi = {10.1126/scisignal.2000037}, issn = {1937-9145}, journal = {Science signaling}, keywords = {bchb-583, systems-biology}, number = {45}, posted-at = {2009-03-15 18:45:17}, priority = {2}, title = {New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening.}, url = {http://dx.doi.org/10.1126/scisignal.2000037}, volume = {1}, year = {2008} }

TY - JOUR ID - citeulike:4179613 L3 - citeulike-article-id:4179613 N2 - The identification and characterization of previously unidentified signal transduction molecules has expanded our understanding of biological systems and facilitated the development of mechanism-based therapeutics. We present a highly validated small interfering RNA (siRNA) screen that functionally annotates the human genome for modulation of the Wnt/beta-catenin signal transduction pathway. Merging these functional data with an extensive Wnt/beta-catenin protein interaction network produces an integrated physical and functional map of the pathway. The power of this approach is illustrated by the positioning of siRNA screen hits into discrete physical complexes of proteins. Similarly, this approach allows one to filter discoveries made through protein-protein interaction screens for functional contribution to the phenotype of interest. Using this methodology, we characterized AGGF1 as a nuclear chromatin-associated protein that participates in beta-catenin-mediated transcription in human colon cancer cells. IS - 45 JF - Science signaling SN - 1937-9145 TI - New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening. VL - 1 KW - bchb-583 KW - systems-biology AU - Major, MB AU - Roberts, BS AU - Berndt, JD AU - Marine, S AU - Anastas, J AU - Chung, N AU - Ferrer, M AU - Yi, X AU - Stoick-Cooper, CL AU - von Haller, PD AU - Kategaya, L AU - Chien, A AU - Angers, S AU - MacCoss, M AU - Cleary, MA AU - Arthur, WT AU - Moon, RT PY - 2008/// UR - http://dx.doi.org/10.1126/scisignal.2000037 ER -