Evolution of the Antibiotic Resistance Protein, FosA, Is Linked to a Catalytically Promiscuous Progenitor† Export

@article{citeulike:4052853, abstract = {doi: 10.1021/bi900078q The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosXMl) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activity toward 1 and does not confer resistance to the antibiotic. In vitro homologous recombination of the mlr3345 and pa1129 genes (a fosA gene from Pseudomonas aeruginosa that does confer robust resistance to 1) produces recombinant proteins that confer resistance to 1 and indicate that the FosA resistance proteins are functionally and genetically related to mlr3345.}, author = {Brown, Daniel W. and Schaab, Matthew R. and Birmingham, William R. and Armstrong, Richard N.}, citeulike-article-id = {4052853}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/bi900078q}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/bi900078q}, doi = {10.1021/bi900078q}, journal = {Biochemistry}, keywords = {antibiotic, enzymology, evolution, fosfomycin, mesorhizobium, protein, resistance}, number = {0}, posted-at = {2009-02-15 02:49:15}, priority = {2}, title = {Evolution of the Antibiotic Resistance Protein, FosA, Is Linked to a Catalytically Promiscuous Progenitor{\dag}}, url = {http://dx.doi.org/10.1021/bi900078q}, volume = {0}, year = {0000} }

TY - JOUR ID - citeulike:4052853 L3 - citeulike-article-id:4052853 N2 - doi: 10.1021/bi900078q The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosXMl) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activity toward 1 and does not confer resistance to the antibiotic. In vitro homologous recombination of the mlr3345 and pa1129 genes (a fosA gene from Pseudomonas aeruginosa that does confer robust resistance to 1) produces recombinant proteins that confer resistance to 1 and indicate that the FosA resistance proteins are functionally and genetically related to mlr3345. IS - 0 JF - Biochemistry TI - Evolution of the Antibiotic Resistance Protein, FosA, Is Linked to a Catalytically Promiscuous Progenitor† VL - 0 KW - antibiotic KW - enzymology KW - evolution KW - fosfomycin KW - mesorhizobium KW - protein KW - resistance AU - Brown, Daniel AU - Schaab, Matthew AU - Birmingham, William AU - Armstrong, Richard PY - 2000/// UR - http://dx.doi.org/10.1021/bi900078q ER -