Rapid inexpensive genome-wide association using pooled whole blood. Export

@article{citeulike:5887965, abstract = {Genome-wide association studies (GWAS) have now successfully identified important genetic variants associated with many human traits and diseases. The high cost of genotyping arrays in large data sets remains the major barrier to wider utilization of GWAS. We have developed a novel method in which whole blood from cases and controls, respectively, is pooled prior to DNA extraction for genotyping. We demonstrate proof of principle by clearly identifying the associated variants for eye color, age-related macular degeneration, and pseudoexfoliation syndrome in cohorts not previously studied. Blood pooling has the potential to reduce GWAS cost by several orders of magnitude and dramatically shorten gene discovery time. This method has profound implications for translation of modern genetic approaches to a multitude of diseases and traits yet to be analyzed by GWAS, and will enable developing nations to participate in GWAS.}, author = {Craig, Jamie E. and Hewitt, Alex W. and McMellon, Amy E. and Henders, Anjali K. and Ma, Lingjun and Wallace, Leanne and Sharma, Shiwani and Burdon, Kathryn P. and Visscher, Peter M. and Montgomery, Grant W. and Macgregor, Stuart}, citeulike-article-id = {5887965}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.094680.109}, citeulike-linkout-1 = {http://genome.cshlp.org/content/early/2009/10/02/gr.094680.109.abstract}, citeulike-linkout-2 = {http://genome.cshlp.org/content/early/2009/10/02/gr.094680.109.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19801603}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19801603}, day = {3}, doi = {10.1101/gr.094680.109}, issn = {1549-5477}, journal = {Genome research}, keywords = {blood, cost, gwas, methods}, month = {October}, number = {11}, pages = {2075--2080}, posted-at = {2009-11-02 22:28:07}, priority = {2}, title = {Rapid inexpensive genome-wide association using pooled whole blood.}, url = {http://dx.doi.org/10.1101/gr.094680.109}, volume = {19}, year = {2009} }

TY - JOUR ID - citeulike:5887965 L3 - citeulike-article-id:5887965 N2 - Genome-wide association studies (GWAS) have now successfully identified important genetic variants associated with many human traits and diseases. The high cost of genotyping arrays in large data sets remains the major barrier to wider utilization of GWAS. We have developed a novel method in which whole blood from cases and controls, respectively, is pooled prior to DNA extraction for genotyping. We demonstrate proof of principle by clearly identifying the associated variants for eye color, age-related macular degeneration, and pseudoexfoliation syndrome in cohorts not previously studied. Blood pooling has the potential to reduce GWAS cost by several orders of magnitude and dramatically shorten gene discovery time. This method has profound implications for translation of modern genetic approaches to a multitude of diseases and traits yet to be analyzed by GWAS, and will enable developing nations to participate in GWAS. IS - 11 JF - Genome research SN - 1549-5477 EP - 2080 TI - Rapid inexpensive genome-wide association using pooled whole blood. VL - 19 SP - 2075 KW - blood KW - cost KW - gwas KW - methods AU - Craig, Jamie AU - Hewitt, Alex AU - McMellon, Amy AU - Henders, Anjali AU - Ma, Lingjun AU - Wallace, Leanne AU - Sharma, Shiwani AU - Burdon, Kathryn AU - Visscher, Peter AU - Montgomery, Grant AU - Macgregor, Stuart PY - 2009/10/03/ UR - http://dx.doi.org/10.1101/gr.094680.109 ER -