Quantification of Membrane Proteins Using Nonspecific Protease Digestions. Export

@article{citeulike:6090802, abstract = {We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.}, author = {Bendz, Maria and Möller, Mirja Carlsson C. and Arrigoni, Giorgio and Wåhlander, Asa and Stella, Roberto and Cappadona, Salvatore and Levander, Fredrik and Hederstedt, Lars and James, Peter}, citeulike-article-id = {6090802}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/pr900741t}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/pr900741t}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19845334}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19845334}, day = {9}, doi = {10.1021/pr900741t}, issn = {1535-3907}, journal = {Journal of proteome research}, keywords = {mass-spec, membrane, protease, protein, proteomics, quantitation}, month = {November}, number = {0}, posted-at = {2009-11-09 22:13:58}, priority = {2}, title = {Quantification of Membrane Proteins Using Nonspecific Protease Digestions.}, url = {http://dx.doi.org/10.1021/pr900741t}, volume = {0}, year = {2009} }

TY - JOUR ID - citeulike:6090802 L3 - citeulike-article-id:6090802 N2 - We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose. IS - 0 JF - Journal of proteome research SN - 1535-3907 TI - Quantification of Membrane Proteins Using Nonspecific Protease Digestions. VL - 0 KW - mass-spec KW - membrane KW - protease KW - protein KW - proteomics KW - quantitation AU - Bendz, Maria AU - Möller, Mirja Carlsson AU - Arrigoni, Giorgio AU - Wåhlander, Asa AU - Stella, Roberto AU - Cappadona, Salvatore AU - Levander, Fredrik AU - Hederstedt, Lars AU - James, Peter PY - 2009/11/09/ UR - http://dx.doi.org/10.1021/pr900741t ER -