Two-Dimensional Difference Gel Electrophoresis (DiGE) Analysis of Plasmas from Dengue Fever Patients Export

@article{citeulike:6090811, abstract = {Dengue fever is the world's most important arthropod-born viral disease affecting humans. To contribute to a better understanding of its pathogenesis, this study aims to identify proteins differentially expressed in plasmas from severe dengue fever patients relative to healthy donors. The use of 2-D Fluorescence Difference Gel Electrophoresis to analyze plasmas depleted of six high-abundance proteins (albumin, IgG, antitrypsin, IgA, transferrin and haptoglobin) allowed for the detection of 73 differentially expressed protein spots (n = 13, p < 0.01), of which 37 could be identified by mass spectrometry. These 37 spots comprised a total of 14 proteins, as follows: 7 had increased expression in plasmas from dengue fever patients (C1 inhibitor, α1-antichymotrypsin, vitamin D-binding protein, fibrinogen γ-chain, α1-acid glycoprotein, apolipoprotein J and complement component C3c), while 7 others had decreased expression in the same samples (α-2 macroglobulin, prothrombin, histidine-rich glycoprotein, apolipoproteins A-IV and A-I, transthyretin and complement component C3b). The possible involvement of these proteins in the inflammatory process triggered by dengue virus infection and in the repair mechanisms of vascular damage occurring in this pathology is discussed in this study.}, author = {Albuquerque, Lidiane M. and Trugilho, Monique R. O. and Chapeaurouge, Alex and Jurgilas, Patrícia B. and Bozza, Patrícia T. and Bozza, Fernando A. and Perales, Jonas and Neves-Ferreira, Ana G. C.}, citeulike-article-id = {6090811}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/pr900236f}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/pr900236f}, doi = {10.1021/pr900236f}, journal = {Journal of Proteome Research}, keywords = {2d-dige, dengue, disease, human, plasma, proteomics, virus}, number = {0}, posted-at = {2009-11-09 22:20:54}, priority = {2}, title = {Two-Dimensional Difference Gel Electrophoresis (DiGE) Analysis of Plasmas from Dengue Fever Patients}, url = {http://dx.doi.org/10.1021/pr900236f}, volume = {0}, year = {0000} }

TY - JOUR ID - citeulike:6090811 L3 - citeulike-article-id:6090811 N2 - Dengue fever is the world’s most important arthropod-born viral disease affecting humans. To contribute to a better understanding of its pathogenesis, this study aims to identify proteins differentially expressed in plasmas from severe dengue fever patients relative to healthy donors. The use of 2-D Fluorescence Difference Gel Electrophoresis to analyze plasmas depleted of six high-abundance proteins (albumin, IgG, antitrypsin, IgA, transferrin and haptoglobin) allowed for the detection of 73 differentially expressed protein spots (n = 13, p < 0.01), of which 37 could be identified by mass spectrometry. These 37 spots comprised a total of 14 proteins, as follows: 7 had increased expression in plasmas from dengue fever patients (C1 inhibitor, α1-antichymotrypsin, vitamin D-binding protein, fibrinogen γ-chain, α1-acid glycoprotein, apolipoprotein J and complement component C3c), while 7 others had decreased expression in the same samples (α-2 macroglobulin, prothrombin, histidine-rich glycoprotein, apolipoproteins A-IV and A-I, transthyretin and complement component C3b). The possible involvement of these proteins in the inflammatory process triggered by dengue virus infection and in the repair mechanisms of vascular damage occurring in this pathology is discussed in this study. IS - 0 JF - Journal of Proteome Research TI - Two-Dimensional Difference Gel Electrophoresis (DiGE) Analysis of Plasmas from Dengue Fever Patients VL - 0 KW - 2d-dige KW - dengue KW - disease KW - human KW - plasma KW - proteomics KW - virus AU - Albuquerque, Lidiane AU - Trugilho, Monique AU - Chapeaurouge, Alex AU - Jurgilas, Patrícia AU - Bozza, Patrícia AU - Bozza, Fernando AU - Perales, Jonas AU - Neves-Ferreira, Ana PY - 2000/// UR - http://dx.doi.org/10.1021/pr900236f ER -