Metabolomics applied to exhaled breath condensate in childhood asthma. Export

@article{carraro07, abstract = {RATIONALE: Metabolomic analysis provides biochemical profiles of low-molecular-weight endogenous metabolites in biological fluids. OBJECTIVES: The aim of this study was to assess the feasibility of nuclear magnetic resonance (NMR)-based metabolomic analysis applied to exhaled breath condensate ("breathomics"). Information coming from NMR spectra was analyzed with a view to establish the NMR variables that best discriminate between children with asthma and healthy children. METHODS: Twenty-five children with asthma (17 with persistent asthma treated with inhaled corticosteroids, 8 with intermittent asthma inhaled corticosteroid naive; age, 7-15 yr) and 11 healthy age-matched control subjects were enrolled. Every child performed exhaled nitric oxide measurement, spirometry, and condensate collection. Condensate samples were analyzed by means of NMR spectroscopy. Linear and partial least squares discriminant analyses were applied to data obtained from the NMR spectra. MEASUREMENTS AND MAIN RESULTS: The combination of exhaled nitric oxide and FEV(1) discriminates children with asthma and healthy children with a success rate of approximately 81\%, whereas selected signals from NMR spectra offer a slightly better discrimination (approximately 86\%). The selected NMR variables derive from the region of 3.2 to 3.4 ppm, indicative of oxidized compounds, and from the region of 1.7 to 2.2 ppm, indicative of acetylated compounds. CONCLUSIONS: Metabolomics can be applied to exhaled breath condensate, leading to the characterization of airway biochemical fingerprints. The presence of acetylated compounds suggests new metabolic pathways that may have a role in asthma pathophysiology.}, address = {Department of Pediatrics, University of Padova, 35128 Padova, Italy.}, author = {Carraro, Silvia and Rezzi, Serge and Reniero, Fabiano and H\'{e}berger, K\'{a}roly and Giordano, Giuseppe and Zanconato, Stefania and Guillou, Claude and Baraldi, Eugenio}, citeulike-article-id = {2812176}, citeulike-linkout-0 = {http://dx.doi.org/10.1164/rccm.200606-769OC}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17303796}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17303796}, day = {15}, doi = {10.1164/rccm.200606-769OC}, issn = {1073-449X}, journal = {American journal of respiratory and critical care medicine}, keywords = {biomarker, nmr, nmr\_moltwin}, month = {May}, number = {10}, pages = {986--990}, posted-at = {2009-10-10 12:39:26}, priority = {2}, title = {Metabolomics applied to exhaled breath condensate in childhood asthma.}, url = {http://dx.doi.org/10.1164/rccm.200606-769OC}, volume = {175}, year = {2007} }

TY - JOUR ID - carraro07 L3 - citeulike-article-id:2812176 N2 - RATIONALE: Metabolomic analysis provides biochemical profiles of low-molecular-weight endogenous metabolites in biological fluids. OBJECTIVES: The aim of this study was to assess the feasibility of nuclear magnetic resonance (NMR)-based metabolomic analysis applied to exhaled breath condensate ("breathomics"). Information coming from NMR spectra was analyzed with a view to establish the NMR variables that best discriminate between children with asthma and healthy children. METHODS: Twenty-five children with asthma (17 with persistent asthma treated with inhaled corticosteroids, 8 with intermittent asthma inhaled corticosteroid naive; age, 7-15 yr) and 11 healthy age-matched control subjects were enrolled. Every child performed exhaled nitric oxide measurement, spirometry, and condensate collection. Condensate samples were analyzed by means of NMR spectroscopy. Linear and partial least squares discriminant analyses were applied to data obtained from the NMR spectra. MEASUREMENTS AND MAIN RESULTS: The combination of exhaled nitric oxide and FEV(1) discriminates children with asthma and healthy children with a success rate of approximately 81%, whereas selected signals from NMR spectra offer a slightly better discrimination (approximately 86%). The selected NMR variables derive from the region of 3.2 to 3.4 ppm, indicative of oxidized compounds, and from the region of 1.7 to 2.2 ppm, indicative of acetylated compounds. CONCLUSIONS: Metabolomics can be applied to exhaled breath condensate, leading to the characterization of airway biochemical fingerprints. The presence of acetylated compounds suggests new metabolic pathways that may have a role in asthma pathophysiology. IS - 10 JF - American journal of respiratory and critical care medicine SN - 1073-449X AD - Department of Pediatrics, University of Padova, 35128 Padova, Italy. EP - 990 TI - Metabolomics applied to exhaled breath condensate in childhood asthma. VL - 175 SP - 986 KW - biomarker KW - nmr KW - nmr_moltwin AU - Carraro, Silvia AU - Rezzi, Serge AU - Reniero, Fabiano AU - Héberger, Károly AU - Giordano, Giuseppe AU - Zanconato, Stefania AU - Guillou, Claude AU - Baraldi, Eugenio PY - 2007/05/15/ UR - http://dx.doi.org/10.1164/rccm.200606-769OC ER -