Three modes of synaptic vesicular recycling revealed by single-vesicle imaging. Export

@article{citeulike:2909794, abstract = {Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400-860 ms) 'kiss-and-run' mode that has a selective fusion pore; a slow (8-21 s) 'compensatory' mode; and a 'stranded' mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval.}, address = {Neurosciences Program, University of California, San Diego, La Jolla, California 92093, USA.}, author = {Gandhi, Sunil P. and Stevens, Charles F.}, citeulike-article-id = {2909794}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature01677}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12789331}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12789331}, day = {5}, doi = {10.1038/nature01677}, issn = {0028-0836}, journal = {Nature}, keywords = {091107, jc, sungmo}, month = {June}, number = {6940}, pages = {607--613}, posted-at = {2009-11-02 08:30:10}, priority = {2}, title = {Three modes of synaptic vesicular recycling revealed by single-vesicle imaging.}, url = {http://dx.doi.org/10.1038/nature01677}, volume = {423}, year = {2003} }

TY - JOUR ID - citeulike:2909794 L3 - citeulike-article-id:2909794 N2 - Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400-860 ms) 'kiss-and-run' mode that has a selective fusion pore; a slow (8-21 s) 'compensatory' mode; and a 'stranded' mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval. IS - 6940 JF - Nature SN - 0028-0836 AD - Neurosciences Program, University of California, San Diego, La Jolla, California 92093, USA. EP - 613 TI - Three modes of synaptic vesicular recycling revealed by single-vesicle imaging. VL - 423 SP - 607 KW - 091107 KW - jc KW - sungmo AU - Gandhi, Sunil AU - Stevens, Charles PY - 2003/06/05/ UR - http://dx.doi.org/10.1038/nature01677 ER -