Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma Export

@article{citeulike:5022813, abstract = {Verification of candidate biomarkers relies upon specific, quantitative assays optimized for selective detection of target proteins, and is increasingly viewed as a critical step in the discovery pipeline that bridges unbiased biomarker discovery to preclinical validation. Although individual laboratories have demonstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can quantify candidate protein biomarkers in plasma, reproducibility and transferability of these assays between laboratories have not been demonstrated. We describe a multilaboratory study to assess reproducibility, recovery, linear dynamic range and limits of detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC. Using common materials and standardized protocols, we demonstrate that these assays can be highly reproducible within and across laboratories and instrument platforms, and are sensitive to low mug/ml protein concentrations in unfractionated plasma. We provide data and benchmarks against which individual laboratories can compare their performance and evaluate new technologies for biomarker verification in plasma.}, author = {Addona, Terri A. and Abbatiello, Susan E. and Schilling, Birgit and Skates, Steven J. and Mani, D. R. and Bunk, David M. and Spiegelman, Clifford H. and Zimmerman, Lisa J. and Ham, Amy-Joan L. and Keshishian, Hasmik and Hall, Steven C. and Allen, Simon and Blackman, Ronald K. and Borchers, Christoph H. and Buck, Charles and Cardasis, Helene L. and Cusack, Michael P. and Dodder, Nathan G. and Gibson, Bradford W. and Held, Jason M. and Hiltke, Tara and Jackson, Angela and Johansen, Eric B. and Kinsinger, Christopher R. and Li, Jing and Mesri, Mehdi and Neubert, Thomas A. and Niles, Richard K. and Pulsipher, Trenton C. and Ransohoff, David and Rodriguez, Henry and Rudnick, Paul A. and Smith, Derek and Tabb, David L. and Tegeler, Tony J. and Variyath, Asokan M. and Vega-Montoto, Lorenzo J. and Wahlander, Asa and Waldemarson, Sofia and Wang, Mu and Whiteaker, Jeffrey R. and Zhao, Lei and Anderson, N. Leigh and Fisher, Susan J. and Liebler, Daniel C. and Paulovich, Amanda G. and Regnier, Fred E. and Tempst, Paul and Carr, Steven A.}, citeulike-article-id = {5022813}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.1546}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.1546}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19561596}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19561596}, doi = {10.1038/nbt.1546}, issn = {1546-1696}, journal = {Nat Biotech}, keywords = {ms, ocbn, proteomics}, month = {July}, number = {7}, pages = {633--641}, posted-at = {2009-07-03 23:08:15}, priority = {2}, publisher = {Nature Publishing Group}, title = {Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma}, url = {http://dx.doi.org/10.1038/nbt.1546}, volume = {27}, year = {2009} }

TY - JOUR ID - citeulike:5022813 L3 - citeulike-article-id:5022813 N2 - Verification of candidate biomarkers relies upon specific, quantitative assays optimized for selective detection of target proteins, and is increasingly viewed as a critical step in the discovery pipeline that bridges unbiased biomarker discovery to preclinical validation. Although individual laboratories have demonstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can quantify candidate protein biomarkers in plasma, reproducibility and transferability of these assays between laboratories have not been demonstrated. We describe a multilaboratory study to assess reproducibility, recovery, linear dynamic range and limits of detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC. Using common materials and standardized protocols, we demonstrate that these assays can be highly reproducible within and across laboratories and instrument platforms, and are sensitive to low mug/ml protein concentrations in unfractionated plasma. We provide data and benchmarks against which individual laboratories can compare their performance and evaluate new technologies for biomarker verification in plasma. IS - 7 JF - Nat Biotech SN - 1546-1696 EP - 641 TI - Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma PB - Nature Publishing Group VL - 27 SP - 633 KW - ms KW - ocbn KW - proteomics AU - Addona, Terri AU - Abbatiello, Susan AU - Schilling, Birgit AU - Skates, Steven AU - Mani, DR AU - Bunk, David AU - Spiegelman, Clifford AU - Zimmerman, Lisa AU - Ham, Amy-Joan AU - Keshishian, Hasmik AU - Hall, Steven AU - Allen, Simon AU - Blackman, Ronald AU - Borchers, Christoph AU - Buck, Charles AU - Cardasis, Helene AU - Cusack, Michael AU - Dodder, Nathan AU - Gibson, Bradford AU - Held, Jason AU - Hiltke, Tara AU - Jackson, Angela AU - Johansen, Eric AU - Kinsinger, Christopher AU - Li, Jing AU - Mesri, Mehdi AU - Neubert, Thomas AU - Niles, Richard AU - Pulsipher, Trenton AU - Ransohoff, David AU - Rodriguez, Henry AU - Rudnick, Paul AU - Smith, Derek AU - Tabb, David AU - Tegeler, Tony AU - Variyath, Asokan AU - Vega-Montoto, Lorenzo AU - Wahlander, Asa AU - Waldemarson, Sofia AU - Wang, Mu AU - Whiteaker, Jeffrey AU - Zhao, Lei AU - Anderson, Leigh AU - Fisher, Susan AU - Liebler, Daniel AU - Paulovich, Amanda AU - Regnier, Fred AU - Tempst, Paul AU - Carr, Steven PY - 2009/07/28/ UR - http://dx.doi.org/10.1038/nbt.1546 ER -