Intracellular pH in one-cell mouse embryo differs between subcellular compartments and between interphase and mitosis Export

@article{citeulike:1903185, abstract = {The pH-sensitive dual-emission fluorophore SNARF-1 coupled with a laser confocal microspectrofluorimeter was used to measure the internal pH (pHi) in different subcellular and subnuclear compartments of early mouse embryos. By this method we analysed the first cell cycle of naturally fertilised embryos in order to detect possible pHi changes correlated to cellular events, particularly the onset of replication or transcription and the first mitosis. Throughout interphase, significant differences of pHi were observed between cytoplasm and pronuclei, and, even more striking, between these compartments and nucleolus precursor bodies, whose pHi was systematically lower. We could detect significant pHi change neither during the replication phase nor at the onset of zygotic transcription, but pHi increased at the end of the one-cell stage in both cytoplasm and chromatin regions, a process that seemed specifically correlated with mitosis.}, author = {Amirand, Claudine and Mentre, Pascale and van de Geijn, Sjoerd and Waksmundzka, Malgorzata and Debey, Pascale}, citeulike-article-id = {1903185}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0248-4900(00)01080-7}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6VRK-41Y85K6-3/2/e21646dcf1e5a5f508e79d8978bb3c5a}, doi = {10.1016/S0248-4900(00)01080-7}, journal = {Biology of the Cell}, keywords = {nucleosome, nucleus, ph}, month = {September}, number = {6}, pages = {409--419}, posted-at = {2007-11-12 16:19:08}, priority = {0}, title = {Intracellular pH in one-cell mouse embryo differs between subcellular compartments and between interphase and mitosis}, url = {http://dx.doi.org/10.1016/S0248-4900(00)01080-7}, volume = {92}, year = {2000} }

TY - JOUR ID - citeulike:1903185 L3 - citeulike-article-id:1903185 N2 - The pH-sensitive dual-emission fluorophore SNARF-1 coupled with a laser confocal microspectrofluorimeter was used to measure the internal pH (pHi) in different subcellular and subnuclear compartments of early mouse embryos. By this method we analysed the first cell cycle of naturally fertilised embryos in order to detect possible pHi changes correlated to cellular events, particularly the onset of replication or transcription and the first mitosis. Throughout interphase, significant differences of pHi were observed between cytoplasm and pronuclei, and, even more striking, between these compartments and nucleolus precursor bodies, whose pHi was systematically lower. We could detect significant pHi change neither during the replication phase nor at the onset of zygotic transcription, but pHi increased at the end of the one-cell stage in both cytoplasm and chromatin regions, a process that seemed specifically correlated with mitosis. IS - 6 JF - Biology of the Cell EP - 419 TI - Intracellular pH in one-cell mouse embryo differs between subcellular compartments and between interphase and mitosis VL - 92 SP - 409 KW - nucleosome KW - nucleus KW - ph AU - Amirand, Claudine AU - Mentre, Pascale AU - van de Geijn, Sjoerd AU - Waksmundzka, Malgorzata AU - Debey, Pascale PY - 2000/09// UR - http://dx.doi.org/10.1016/S0248-4900(00)01080-7 ER -