Direct Measurements of Base Stacking Interactions in DNA by Single-Molecule Atomic-Force Spectroscopy Export

@article{citeulike:3351992, abstract = {We investigate the elasticity of two types of single-stranded synthetic DNA homopolydeoxynucletides, poly(dA) and poly(dT), by AFM-based single-molecule force spectroscopy. We find that poly(dT) exhibits the expected entropic elasticity behavior, while poly(dA) unexpectedly displays two overstretching transitions in the force-extension relationship. We suggest that these transitions, which occur at \˜23pN and \˜113pN, directly capture, for the first time, the mechanical signature of base-stacking interactions among adenines in DNA, in the absence of base pairing.}, author = {Ke, C. and Humeniuk, M. and S-Gracz, H. and Marszalek, P. E.}, citeulike-article-id = {3351992}, citeulike-linkout-0 = {http://dx.doi.org/10.1103/PhysRevLett.99.018302}, citeulike-linkout-1 = {http://adsabs.harvard.edu/cgi-bin/nph-bib_query?bibcode=2007PhRvL..99a8302K}, doi = {10.1103/PhysRevLett.99.018302}, journal = {Physical Review Letters}, keywords = {dna\_bending, dna\_stacking, dna\_stretching}, month = {July}, number = {1}, pages = {018302+}, posted-at = {2008-09-29 10:57:07}, priority = {2}, title = {Direct Measurements of Base Stacking Interactions in DNA by Single-Molecule Atomic-Force Spectroscopy}, url = {http://dx.doi.org/10.1103/PhysRevLett.99.018302}, volume = {99}, year = {2007} }

TY - JOUR ID - citeulike:3351992 L3 - citeulike-article-id:3351992 N2 - We investigate the elasticity of two types of single-stranded synthetic DNA homopolydeoxynucletides, poly(dA) and poly(dT), by AFM-based single-molecule force spectroscopy. We find that poly(dT) exhibits the expected entropic elasticity behavior, while poly(dA) unexpectedly displays two overstretching transitions in the force-extension relationship. We suggest that these transitions, which occur at ˜23pN and ˜113pN, directly capture, for the first time, the mechanical signature of base-stacking interactions among adenines in DNA, in the absence of base pairing. IS - 1 JF - Physical Review Letters TI - Direct Measurements of Base Stacking Interactions in DNA by Single-Molecule Atomic-Force Spectroscopy VL - 99 SP - 018302 KW - dna_bending KW - dna_stacking KW - dna_stretching AU - Ke, C AU - Humeniuk, M AU - S-Gracz, H AU - Marszalek, PE PY - 2007/July// UR - http://dx.doi.org/10.1103/PhysRevLett.99.018302 ER -