Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles Export

@article{citeulike:4842666, abstract = {We use cryo-electron microscopy (cryo-EM) to study the 3D shapes of 94-bp-long DNA minicircles and address the question of whether cyclization of such short DNA molecules necessitates the formation of sharp, localized kinks in DNA or whether the necessary bending can be redistributed and accomplished within the limits of the elastic, standard model of DNA flexibility. By comparing the shapes of covalently closed, nicked and gapped DNA minicircles, we conclude that 94-bp-long covalently closed and nicked DNA minicircles do not show sharp kinks while gapped DNA molecules, containing very flexible single-stranded regions, do show sharp kinks. We corroborate the results of cryo-EM studies by using Bal31 nuclease to probe for the existence of kinks in 94-bp-long minicircles. 10.1093/nar/gkp137}, author = {Demurtas, Davide and Amzallag, Arnaud and Rawdon, Eric J. and Maddocks, John H. and Dubochet, Jacques and Stasiak, Andrzej}, citeulike-article-id = {4842666}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkp137}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/37/9/2882}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19282451}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19282451}, day = {1}, doi = {10.1093/nar/gkp137}, journal = {Nucl. Acids Res.}, keywords = {dna, dna\_bending}, month = {May}, number = {9}, pages = {2882--2893}, posted-at = {2009-06-14 07:37:48}, priority = {2}, title = {Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles}, url = {http://dx.doi.org/10.1093/nar/gkp137}, volume = {37}, year = {2009} }

TY - JOUR ID - citeulike:4842666 L3 - citeulike-article-id:4842666 N2 - We use cryo-electron microscopy (cryo-EM) to study the 3D shapes of 94-bp-long DNA minicircles and address the question of whether cyclization of such short DNA molecules necessitates the formation of sharp, localized kinks in DNA or whether the necessary bending can be redistributed and accomplished within the limits of the elastic, standard model of DNA flexibility. By comparing the shapes of covalently closed, nicked and gapped DNA minicircles, we conclude that 94-bp-long covalently closed and nicked DNA minicircles do not show sharp kinks while gapped DNA molecules, containing very flexible single-stranded regions, do show sharp kinks. We corroborate the results of cryo-EM studies by using Bal31 nuclease to probe for the existence of kinks in 94-bp-long minicircles. 10.1093/nar/gkp137 IS - 9 JF - Nucl. Acids Res. EP - 2893 TI - Bending modes of DNA directly addressed by cryo-electron microscopy of DNA minicircles VL - 37 SP - 2882 KW - dna KW - dna_bending AU - Demurtas, Davide AU - Amzallag, Arnaud AU - Rawdon, Eric AU - Maddocks, John AU - Dubochet, Jacques AU - Stasiak, Andrzej PY - 2009/05/01/ UR - http://dx.doi.org/10.1093/nar/gkp137 ER -