Interaction of spermine and DNA Export

@article{citeulike:6096403, abstract = {The effect of spermine upon the denaturation temperature (Tm) of DNA's of various base compositions has been found to depend upon both the base composition of the DNA and the pH of the solution. Measurement of the hydrogen ion titration curve of spermine as a function of temperature reveals that the net charge of the spermine molecule is undergoing a rapid change with temperature in the range of temperatures at which DNA denatures. Since the value of Tm depends upon base composition, the correlation of the effect of spermine upon Tm with the base composition of the DNA used may be explainable in terms of the changing degree of ionization of spermine. The binding of spermine to native DNA has also been studied by dialysis equilibrium. There is no significant variation either in the number of strongly binding sites or strength of binding with base composition. It is concluded that there is no evidence of correlation between the binding of spermine and the base composition of DNA.}, address = {Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014}, author = {Hirschman, Shalom Z. and Leng, Marc and Felsenfeld, Gary}, citeulike-article-id = {6096403}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/bip.1967.360050209}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/107584410/ABSTRACT}, doi = {10.1002/bip.1967.360050209}, issn = {1097-0282}, journal = {Biopolymers}, keywords = {dna, dna\_ions, spermine}, number = {2}, pages = {227--233}, posted-at = {2009-11-11 03:09:36}, priority = {2}, title = {Interaction of spermine and DNA}, url = {http://dx.doi.org/10.1002/bip.1967.360050209}, volume = {5}, year = {1967} }

TY - JOUR ID - citeulike:6096403 L3 - citeulike-article-id:6096403 N2 - The effect of spermine upon the denaturation temperature (Tm) of DNA's of various base compositions has been found to depend upon both the base composition of the DNA and the pH of the solution. Measurement of the hydrogen ion titration curve of spermine as a function of temperature reveals that the net charge of the spermine molecule is undergoing a rapid change with temperature in the range of temperatures at which DNA denatures. Since the value of Tm depends upon base composition, the correlation of the effect of spermine upon Tm with the base composition of the DNA used may be explainable in terms of the changing degree of ionization of spermine. The binding of spermine to native DNA has also been studied by dialysis equilibrium. There is no significant variation either in the number of strongly binding sites or strength of binding with base composition. It is concluded that there is no evidence of correlation between the binding of spermine and the base composition of DNA. IS - 2 JF - Biopolymers SN - 1097-0282 AD - Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014 EP - 233 TI - Interaction of spermine and DNA VL - 5 SP - 227 KW - dna KW - dna_ions KW - spermine AU - Hirschman, Shalom AU - Leng, Marc AU - Felsenfeld, Gary PY - 1967/// UR - http://dx.doi.org/10.1002/bip.1967.360050209 ER -