Intrinsic disorder and coiled-coil formation in prostate apoptosis response factor 4. Export

@article{citeulike:5022424, abstract = {Prostate apoptosis response factor-4 (Par-4) is an ubiquitously expressed pro-apoptotic and tumour suppressive protein that can both activate cell-death mechanisms and inhibit pro-survival factors. Par-4 contains a highly conserved coiled-coil region that serves as the primary recognition domain for a large number of binding partners. Par-4 is also tightly regulated by the aforementioned binding partners and by post-translational modifications. Biophysical data obtained in the present study indicate that Par-4 primarily comprises an intrinsically disordered protein. Bioinformatic analysis of the highly conserved Par-4 reveals low sequence complexity and enrichment in polar and charged amino acids. The high proteolytic susceptibility and an increased hydrodynamic radius are consistent with a largely extended structure in solution. Spectroscopic measurements using CD and NMR also reveal characteristic features of intrinsic disorder. Under physiological conditions, the data obtained show that Par-4 self-associates via the C-terminal domain, forming a coiled-coil. Interruption of self-association by urea also resulted in loss of secondary structure. These results are consistent with the stabilization of the coiled-coil motif through an intramolecular association.}, author = {Libich, David S. and Schwalbe, Martin and Kate, Sachin and Venugopal, Hariprasad and Claridge, Jolyon K. and Edwards, Patrick J. and Dutta, Kaushik and Pascal, Steven M.}, citeulike-article-id = {5022424}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1742-4658.2009.07087.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/ejb/2009/00000276/00000014/art00003}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19490121}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19490121}, doi = {10.1111/j.1742-4658.2009.07087.x}, issn = {1742-4658}, journal = {The FEBS journal}, keywords = {coiled\_coil, lox-1, neck\_domain}, month = {July}, number = {14}, pages = {3710--3728}, posted-at = {2009-10-21 19:03:53}, priority = {2}, publisher = {Blackwell Publishing}, title = {Intrinsic disorder and coiled-coil formation in prostate apoptosis response factor 4.}, url = {http://dx.doi.org/10.1111/j.1742-4658.2009.07087.x}, volume = {276}, year = {2009} }

TY - JOUR ID - citeulike:5022424 L3 - citeulike-article-id:5022424 N2 - Prostate apoptosis response factor-4 (Par-4) is an ubiquitously expressed pro-apoptotic and tumour suppressive protein that can both activate cell-death mechanisms and inhibit pro-survival factors. Par-4 contains a highly conserved coiled-coil region that serves as the primary recognition domain for a large number of binding partners. Par-4 is also tightly regulated by the aforementioned binding partners and by post-translational modifications. Biophysical data obtained in the present study indicate that Par-4 primarily comprises an intrinsically disordered protein. Bioinformatic analysis of the highly conserved Par-4 reveals low sequence complexity and enrichment in polar and charged amino acids. The high proteolytic susceptibility and an increased hydrodynamic radius are consistent with a largely extended structure in solution. Spectroscopic measurements using CD and NMR also reveal characteristic features of intrinsic disorder. Under physiological conditions, the data obtained show that Par-4 self-associates via the C-terminal domain, forming a coiled-coil. Interruption of self-association by urea also resulted in loss of secondary structure. These results are consistent with the stabilization of the coiled-coil motif through an intramolecular association. IS - 14 JF - The FEBS journal SN - 1742-4658 EP - 3728 TI - Intrinsic disorder and coiled-coil formation in prostate apoptosis response factor 4. PB - Blackwell Publishing VL - 276 SP - 3710 KW - coiled_coil KW - lox-1 KW - neck_domain AU - Libich, David AU - Schwalbe, Martin AU - Kate, Sachin AU - Venugopal, Hariprasad AU - Claridge, Jolyon AU - Edwards, Patrick AU - Dutta, Kaushik AU - Pascal, Steven PY - 2009/07// UR - http://dx.doi.org/10.1111/j.1742-4658.2009.07087.x ER -