Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis. Export

@article{citeulike:6059937, abstract = {We report the occurrence of an isomerase with a putative (betaalpha)(8)-barrel structure involved in both histidine and trypto-phan biosynthesis in Streptomyces coelicolor A3(2) and Mycobacterium tuberculosis HR37Rv. Deletion of a hisA homologue (SCO2050) putatively encoding N'-[(5'-phosphoribosyl)-formimino]-5 amino-imidazole-4-carboxamide ribonucleotide isomerase from the chromosome of S. coelicolor A3(2) generated a double auxotrophic mutant for histidine and tryptophan. The bifunctional gene SCO2050 and its orthologue Rv1603 from M. tuberculosis complemented both hisA and trpF mutants of Escherichia coli. Expression of the E. coli trpF gene in the S. coelicolor mutant only complemented the tryptophan auxo-trophy, and the hisA gene only complemented the histidine auxotrophy. The discovery of this enzyme, which has a broad-substrate specificity, has implications for the evolution of metabolic pathways and may prove to be important for understanding the evolution of the (betaalpha)(8)-barrels.}, author = {Barona-G\'{o}mez, Francisco and Hodgson, David A.}, citeulike-article-id = {6059937}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/sj.embor.embor771}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12634849}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12634849}, doi = {10.1038/sj.embor.embor771}, issn = {1469-221X}, journal = {EMBO reports}, keywords = {barrel, enzyme, evolution, isomerase, promiscuity}, month = {March}, number = {3}, pages = {296--300}, posted-at = {2009-11-03 11:17:29}, priority = {2}, title = {Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis.}, url = {http://dx.doi.org/10.1038/sj.embor.embor771}, volume = {4}, year = {2003} }

TY - JOUR ID - citeulike:6059937 L3 - citeulike-article-id:6059937 N2 - We report the occurrence of an isomerase with a putative (betaalpha)(8)-barrel structure involved in both histidine and trypto-phan biosynthesis in Streptomyces coelicolor A3(2) and Mycobacterium tuberculosis HR37Rv. Deletion of a hisA homologue (SCO2050) putatively encoding N'-[(5'-phosphoribosyl)-formimino]-5 amino-imidazole-4-carboxamide ribonucleotide isomerase from the chromosome of S. coelicolor A3(2) generated a double auxotrophic mutant for histidine and tryptophan. The bifunctional gene SCO2050 and its orthologue Rv1603 from M. tuberculosis complemented both hisA and trpF mutants of Escherichia coli. Expression of the E. coli trpF gene in the S. coelicolor mutant only complemented the tryptophan auxo-trophy, and the hisA gene only complemented the histidine auxotrophy. The discovery of this enzyme, which has a broad-substrate specificity, has implications for the evolution of metabolic pathways and may prove to be important for understanding the evolution of the (betaalpha)(8)-barrels. IS - 3 JF - EMBO reports SN - 1469-221X EP - 300 TI - Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis. VL - 4 SP - 296 KW - barrel KW - enzyme KW - evolution KW - isomerase KW - promiscuity AU - Barona-Gómez, Francisco AU - Hodgson, David PY - 2003/03// UR - http://dx.doi.org/10.1038/sj.embor.embor771 ER -