Inter- and intramolecular contacts in a membrane protein/surfactant complex observed by heteronuclear dipole-to-dipole cross-relaxation. Export

@article{citeulike:3822484, abstract = {Heteronuclear dipole-to-dipole cross-relaxation has been applied to exploring intermolecular interactions and intramolecular spatial proximities in a large supramolecular structure comprised of a beta-barrel membrane protein, OmpX, in complex with a polymeric surfactant, amphipol A8-35. The experiments, performed in either the laboratory or the rotating frame, reveal the existence of intermolecular contacts between aromatic amino acids and specific groups of the polymer, in addition to intra-protein dipolar interactions, some of them involving carbonyl carbons. This study opens the perspective of collecting by NMR spectroscopy a new kind of through-space structural information involving aromatic and carbonyl (13)C atoms of large proteins.}, author = {Catoire, Laurent J J. and Zoonens, Manuela and Heijenoort, Carine van V. and Giusti, Fabrice and Popot, Jean-Luc L. and Guittet, Eric}, citeulike-article-id = {3822484}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jmr.2008.11.017}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19101186}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19101186}, day = {6}, doi = {10.1016/j.jmr.2008.11.017}, issn = {1557-8968}, journal = {Journal of magnetic resonance (San Diego, Calif. : 1997)}, keywords = {bacteria, lipid, membrane, nmr, outer\_membrane, protein, structure}, month = {December}, posted-at = {2008-12-24 00:17:39}, priority = {2}, title = {Inter- and intramolecular contacts in a membrane protein/surfactant complex observed by heteronuclear dipole-to-dipole cross-relaxation.}, url = {http://dx.doi.org/10.1016/j.jmr.2008.11.017}, year = {2008} }

TY - JOUR ID - citeulike:3822484 L3 - citeulike-article-id:3822484 N2 - Heteronuclear dipole-to-dipole cross-relaxation has been applied to exploring intermolecular interactions and intramolecular spatial proximities in a large supramolecular structure comprised of a beta-barrel membrane protein, OmpX, in complex with a polymeric surfactant, amphipol A8-35. The experiments, performed in either the laboratory or the rotating frame, reveal the existence of intermolecular contacts between aromatic amino acids and specific groups of the polymer, in addition to intra-protein dipolar interactions, some of them involving carbonyl carbons. This study opens the perspective of collecting by NMR spectroscopy a new kind of through-space structural information involving aromatic and carbonyl (13)C atoms of large proteins. JF - Journal of magnetic resonance (San Diego, Calif. : 1997) SN - 1557-8968 TI - Inter- and intramolecular contacts in a membrane protein/surfactant complex observed by heteronuclear dipole-to-dipole cross-relaxation. KW - bacteria KW - lipid KW - membrane KW - nmr KW - outer_membrane KW - protein KW - structure AU - Catoire, Laurent J AU - Zoonens, Manuela AU - Heijenoort, Carine van AU - Giusti, Fabrice AU - Popot, Jean-Luc AU - Guittet, Eric PY - 2008/12/06/ UR - http://dx.doi.org/10.1016/j.jmr.2008.11.017 ER -