An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen Export

@article{citeulike:5004467, abstract = {Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange. 10.1126/science.1175088}, author = {Kornmann, Benoit and Currie, Erin and Collins, Sean R. and Schuldiner, Maya and Nunnari, Jodi and Weissman, Jonathan S. and Walter, Peter}, citeulike-article-id = {5004467}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1175088}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/325/5939/477}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19556461}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19556461}, doi = {10.1126/science.1175088}, issn = {1095-9203}, journal = {Science}, keywords = {er, lipid, mdm10, mdm12, mitochondria, mmm1, transport}, month = {July}, number = {5939}, pages = {477--481}, posted-at = {2009-06-29 01:12:06}, priority = {2}, title = {An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen}, url = {http://dx.doi.org/10.1126/science.1175088}, volume = {325}, year = {2009} }

TY - JOUR ID - citeulike:5004467 L3 - citeulike-article-id:5004467 N2 - Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange. 10.1126/science.1175088 IS - 5939 JF - Science SN - 1095-9203 EP - 481 TI - An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen VL - 325 SP - 477 KW - er KW - lipid KW - mdm10 KW - mdm12 KW - mitochondria KW - mmm1 KW - transport AU - Kornmann, Benoit AU - Currie, Erin AU - Collins, Sean AU - Schuldiner, Maya AU - Nunnari, Jodi AU - Weissman, Jonathan AU - Walter, Peter PY - 2009/07/24/ UR - http://dx.doi.org/10.1126/science.1175088 ER -