PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Export

@article{citeulike:683079, abstract = {DNA microarrays can be used to identify gene expression changes characteristic of human disease. This is challenging, however, when relevant differences are subtle at the level of individual genes. We introduce an analytical strategy, Gene Set Enrichment Analysis, designed to detect modest but coordinate changes in the expression of groups of functionally related genes. Using this approach, we identify a set of genes involved in oxidative phosphorylation whose expression is coordinately decreased in human diabetic muscle. Expression of these genes is high at sites of insulin-mediated glucose disposal, activated by PGC-1alpha and correlated with total-body aerobic capacity. Our results associate this gene set with clinically important variation in human metabolism and illustrate the value of pathway relationships in the analysis of genomic profiling experiments.}, address = {Whitehead Institute/MIT Center for Genome Research, Cambridge, Massachusetts, USA.}, author = {Mootha, Vamsi K. and Lindgren, Cecilia M. and Eriksson, Karl-Fredrik F. and Subramanian, Aravind and Sihag, Smita and Lehar, Joseph and Puigserver, Pere and Carlsson, Emma and Ridderstr\r{a}le, Martin and Laurila, Esa and Houstis, Nicholas and Daly, Mark J. and Patterson, Nick and Mesirov, Jill P. and Golub, Todd R. and Tamayo, Pablo and Spiegelman, Bruce and Lander, Eric S. and Hirschhorn, Joel N. and Altshuler, David and Groop, Leif C.}, citeulike-article-id = {683079}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng1180}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng1180}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12808457}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12808457}, doi = {10.1038/ng1180}, issn = {1061-4036}, journal = {Nature genetics}, keywords = {algorithm, gene-expression, gene-profiling, gene-set-enrichment-analysis, microarray, statistical-testing, statistics}, month = {July}, number = {3}, pages = {267--273}, posted-at = {2009-03-20 11:20:02}, priority = {0}, title = {PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes.}, url = {http://dx.doi.org/10.1038/ng1180}, volume = {34}, year = {2003} }

TY - JOUR ID - citeulike:683079 L3 - citeulike-article-id:683079 N2 - DNA microarrays can be used to identify gene expression changes characteristic of human disease. This is challenging, however, when relevant differences are subtle at the level of individual genes. We introduce an analytical strategy, Gene Set Enrichment Analysis, designed to detect modest but coordinate changes in the expression of groups of functionally related genes. Using this approach, we identify a set of genes involved in oxidative phosphorylation whose expression is coordinately decreased in human diabetic muscle. Expression of these genes is high at sites of insulin-mediated glucose disposal, activated by PGC-1alpha and correlated with total-body aerobic capacity. Our results associate this gene set with clinically important variation in human metabolism and illustrate the value of pathway relationships in the analysis of genomic profiling experiments. IS - 3 JF - Nature genetics SN - 1061-4036 AD - Whitehead Institute/MIT Center for Genome Research, Cambridge, Massachusetts, USA. EP - 273 TI - PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. VL - 34 SP - 267 KW - algorithm KW - gene-expression KW - gene-profiling KW - gene-set-enrichment-analysis KW - microarray KW - statistical-testing KW - statistics AU - Mootha, Vamsi AU - Lindgren, Cecilia AU - Eriksson, Karl-Fredrik AU - Subramanian, Aravind AU - Sihag, Smita AU - Lehar, Joseph AU - Puigserver, Pere AU - Carlsson, Emma AU - Ridderstråle, Martin AU - Laurila, Esa AU - Houstis, Nicholas AU - Daly, Mark AU - Patterson, Nick AU - Mesirov, Jill AU - Golub, Todd AU - Tamayo, Pablo AU - Spiegelman, Bruce AU - Lander, Eric AU - Hirschhorn, Joel AU - Altshuler, David AU - Groop, Leif PY - 2003/07// UR - http://dx.doi.org/10.1038/ng1180 ER -