EpsteinâBarr virus and virus human protein interaction maps Export

@article{citeulike:1276877, abstract = {10.1073/pnas.0702332104 A comprehensive mapping of interactions among Epstein\^{a}€“Barr virus (EBV) proteins and interactions of EBV proteins with human proteins should provide specific hypotheses and a broad perspective on EBV strategies for replication and persistence. Interactions of EBV proteins with each other and with human proteins were assessed by using a stringent high-throughput yeast two-hybrid system. Overall, 43 interactions between EBV proteins and 173 interactions between EBV and human proteins were identified. EBV\^{a}€“EBV and EBV\^{a}€“human protein interaction, or \^{a}€œinteractome\^{a}€ maps provided a framework for hypotheses of protein function. For example, LF2, an EBV protein of unknown function interacted with the EBV immediate early R transactivator (Rta) and was found to inhibit Rta transactivation. From a broader perspective, EBV genes can be divided into two evolutionary classes, \^{a}€œcore\^{a}€ genes, which are conserved across all herpesviruses and subfamily specific, or \^{a}€œnoncore\^{a}€ genes. Our EBV\^{a}€“EBV interactome map is enriched for interactions among proteins in the same evolutionary class. Furthermore, human proteins targeted by EBV proteins were enriched for highly connected or \^{a}€œhub\^{a}€ proteins and for proteins with relatively short paths to all other proteins in the human interactome network. Targeting of hubs might be an efficient mechanism for EBV reorganization of cellular processes.}, address = {Program in Virology, Departments of Medicine and Microbiology and Molecular Genetics, The Channing Laboratory, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, MA 02115.}, author = {Calderwood, Michael A. and Venkatesan, Kavitha and Xing, Li and Chase, Michael R. and Vazquez, Alexei and Holthaus, Amy M. and Ewence, Alexandra E. and Li, Ning and Hirozane-Kishikawa, Tomoko and Hill, David E. and Vidal, Marc and Kieff, Elliott and Johannsen, Eric}, citeulike-article-id = {1276877}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0702332104}, citeulike-linkout-1 = {http://www.pnas.org/content/104/18/7606.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/104/18/7606.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17446270}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17446270}, day = {1}, doi = {10.1073/pnas.0702332104}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences}, keywords = {ebv, edward, network, protein-interaction, virus}, month = {May}, number = {18}, pages = {7606--7611}, posted-at = {2009-10-08 23:19:05}, priority = {2}, title = {Epstein\^{a}€“Barr virus and virus human protein interaction maps}, url = {http://dx.doi.org/10.1073/pnas.0702332104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1276877 L3 - citeulike-article-id:1276877 N2 - 10.1073/pnas.0702332104 A comprehensive mapping of interactions among Epstein–Barr virus (EBV) proteins and interactions of EBV proteins with human proteins should provide specific hypotheses and a broad perspective on EBV strategies for replication and persistence. Interactions of EBV proteins with each other and with human proteins were assessed by using a stringent high-throughput yeast two-hybrid system. Overall, 43 interactions between EBV proteins and 173 interactions between EBV and human proteins were identified. EBV–EBV and EBV–human protein interaction, or “interactome” maps provided a framework for hypotheses of protein function. For example, LF2, an EBV protein of unknown function interacted with the EBV immediate early R transactivator (Rta) and was found to inhibit Rta transactivation. From a broader perspective, EBV genes can be divided into two evolutionary classes, “core” genes, which are conserved across all herpesviruses and subfamily specific, or “noncore” genes. Our EBV–EBV interactome map is enriched for interactions among proteins in the same evolutionary class. Furthermore, human proteins targeted by EBV proteins were enriched for highly connected or “hub” proteins and for proteins with relatively short paths to all other proteins in the human interactome network. Targeting of hubs might be an efficient mechanism for EBV reorganization of cellular processes. IS - 18 JF - Proceedings of the National Academy of Sciences SN - 0027-8424 AD - Program in Virology, Departments of Medicine and Microbiology and Molecular Genetics, The Channing Laboratory, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, MA 02115. EP - 7611 TI - Epstein–Barr virus and virus human protein interaction maps VL - 104 SP - 7606 KW - ebv KW - edward KW - network KW - protein-interaction KW - virus AU - Calderwood, Michael AU - Venkatesan, Kavitha AU - Xing, Li AU - Chase, Michael AU - Vazquez, Alexei AU - Holthaus, Amy AU - Ewence, Alexandra AU - Li, Ning AU - Hirozane-Kishikawa, Tomoko AU - Hill, David AU - Vidal, Marc AU - Kieff, Elliott AU - Johannsen, Eric PY - 2007/05/01/ UR - http://dx.doi.org/10.1073/pnas.0702332104 ER -