MicroRNA-206 Targets notch3, Activates Apoptosis, and Inhibits Tumor Cell Migration and Focus Formation Export

@article{citeulike:5725234, abstract = {10.1074/jbc.M109.046862 MicroRNAs contribute to cancer development by acting as oncogenes or tumor suppressor genes. However, only a few microRNA target genes were determined. We identified a nearly perfect complementarity between miR-206 and the 3\^{a}€²-untranslated regions of both mouse and human . Expression of miR-206 decreased the luciferase activity dose-dependently when cotransfected with the mouse or human 3\^{a}€²-untranslated region-luciferase reporter containing the miR-206 target site in HeLa cells. This suppression was relieved by deletion and mutation of the miR-206-binding site and was partially recovered by expression of or by a specific inhibitor of miR-206. Interestingly, overexpression of miR-206 decreased the levels of both Notch3 protein and mRNA. Expression of miR-206 markedly induced apoptotic cell death and blocked the anti-apoptotic activity of Notch3. In addition, ectopic expression of miR-206 inhibited HeLa cell migration and focus formation. Therefore, we identified miR-206 as a pro-apoptotic activator of cell death, which was associated with its inhibition of signaling and tumor formation. The inhibition of cancer cell migration and focus formation by miR-206 strongly suggests that miR-206 may function as a novel tumor suppressor.}, author = {Song, Guisheng and Zhang, Yuxia and Wang, Li}, citeulike-article-id = {5725234}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M109.046862}, citeulike-linkout-1 = {http://www.jbc.org/content/284/46/31921.abstract}, citeulike-linkout-2 = {http://www.jbc.org/content/284/46/31921.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19723635}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19723635}, day = {13}, doi = {10.1074/jbc.M109.046862}, issn = {0021-9258}, journal = {Journal of Biological Chemistry}, keywords = {cancer, cell, microrna, song09pdf, tumor}, month = {November}, number = {46}, pages = {31921--31927}, posted-at = {2009-11-10 18:38:35}, priority = {2}, title = {MicroRNA-206 Targets notch3, Activates Apoptosis, and Inhibits Tumor Cell Migration and Focus Formation}, url = {http://dx.doi.org/10.1074/jbc.M109.046862}, volume = {284}, year = {2009} }

TY - JOUR ID - citeulike:5725234 L3 - citeulike-article-id:5725234 N2 - 10.1074/jbc.M109.046862 MicroRNAs contribute to cancer development by acting as oncogenes or tumor suppressor genes. However, only a few microRNA target genes were determined. We identified a nearly perfect complementarity between miR-206 and the 3′-untranslated regions of both mouse and human . Expression of miR-206 decreased the luciferase activity dose-dependently when cotransfected with the mouse or human 3′-untranslated region-luciferase reporter containing the miR-206 target site in HeLa cells. This suppression was relieved by deletion and mutation of the miR-206-binding site and was partially recovered by expression of or by a specific inhibitor of miR-206. Interestingly, overexpression of miR-206 decreased the levels of both Notch3 protein and mRNA. Expression of miR-206 markedly induced apoptotic cell death and blocked the anti-apoptotic activity of Notch3. In addition, ectopic expression of miR-206 inhibited HeLa cell migration and focus formation. Therefore, we identified miR-206 as a pro-apoptotic activator of cell death, which was associated with its inhibition of signaling and tumor formation. The inhibition of cancer cell migration and focus formation by miR-206 strongly suggests that miR-206 may function as a novel tumor suppressor. IS - 46 JF - Journal of Biological Chemistry SN - 0021-9258 EP - 31927 TI - MicroRNA-206 Targets notch3, Activates Apoptosis, and Inhibits Tumor Cell Migration and Focus Formation VL - 284 SP - 31921 KW - cancer KW - cell KW - microrna KW - song09pdf KW - tumor AU - Song, Guisheng AU - Zhang, Yuxia AU - Wang, Li PY - 2009/11/13/ UR - http://dx.doi.org/10.1074/jbc.M109.046862 ER -