Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules Export

@article{citeulike:5770575, abstract = {It is widely accepted that tissue differentiation and morphogenesis in multicellular organisms are regulated by tightly controlled concentration gradients of morphogens1, 2. How exactly these gradients are formed, however, remains unclear3, 4, 5, 6, 7, 8, 9, 10, 11, 12. Here we show that Fgf8 morphogen gradients in living zebrafish embryos are established and maintained by two essential factors: fast, free diffusion of single molecules away from the source through extracellular space, and a sink function of the receiving cells, regulated by receptor-mediated endocytosis. Evidence is provided by directly examining single molecules of Fgf8 in living tissue by fluorescence correlation spectroscopy, quantifying their local mobility and concentration with high precision. By changing the degree of uptake of Fgf8 into its target cells, we are able to alter the shape of the Fgf8 gradient. Our results demonstrate that a freely diffusing morphogen can set up concentration gradients in a complex multicellular tissue by a simple source-sink mechanism.}, author = {Yu, Shuizi R. and Burkhardt, Markus and Nowak, Matthias and Ries, Jonas and Petrasek, Zdenek and Scholpp, Steffen and Schwille, Petra and Brand, Michael}, citeulike-article-id = {5770575}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature08391}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature08391}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19741606}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19741606}, day = {09}, doi = {10.1038/nature08391}, issn = {0028-0836}, journal = {Nature}, keywords = {cell, fcs, fgf8, gradient, solution, yu09pdf}, month = {September}, number = {7263}, pages = {533--536}, posted-at = {2009-09-22 20:15:32}, priority = {2}, publisher = {Nature Publishing Group}, title = {Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules}, url = {http://dx.doi.org/10.1038/nature08391}, volume = {461}, year = {2009} }

TY - JOUR ID - citeulike:5770575 L3 - citeulike-article-id:5770575 N2 - It is widely accepted that tissue differentiation and morphogenesis in multicellular organisms are regulated by tightly controlled concentration gradients of morphogens1, 2. How exactly these gradients are formed, however, remains unclear3, 4, 5, 6, 7, 8, 9, 10, 11, 12. Here we show that Fgf8 morphogen gradients in living zebrafish embryos are established and maintained by two essential factors: fast, free diffusion of single molecules away from the source through extracellular space, and a sink function of the receiving cells, regulated by receptor-mediated endocytosis. Evidence is provided by directly examining single molecules of Fgf8 in living tissue by fluorescence correlation spectroscopy, quantifying their local mobility and concentration with high precision. By changing the degree of uptake of Fgf8 into its target cells, we are able to alter the shape of the Fgf8 gradient. Our results demonstrate that a freely diffusing morphogen can set up concentration gradients in a complex multicellular tissue by a simple source-sink mechanism. IS - 7263 JF - Nature SN - 0028-0836 EP - 536 TI - Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules PB - Nature Publishing Group VL - 461 SP - 533 KW - cell KW - fcs KW - fgf8 KW - gradient KW - solution KW - yu09pdf AU - Yu, Shuizi AU - Burkhardt, Markus AU - Nowak, Matthias AU - Ries, Jonas AU - Petrasek, Zdenek AU - Scholpp, Steffen AU - Schwille, Petra AU - Brand, Michael PY - 2009/09/09/ UR - http://dx.doi.org/10.1038/nature08391 ER -