Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level Export

@article{citeulike:5977599, abstract = {10.1073/pnas.0907367106 The use of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of \^{a}€œplasmon rulers\^{a}€ to observe conformational changes of single biomolecules in vitro. Plasmon rulers provide robust signals without photobleaching or blinking. Here, we show the first application of plasmon rulers to in vivo studies to observe very long trajectories of single biomolecules in live cells. We present a unique type of plasmon ruler comprised of peptide-linked gold nanoparticle satellites around a core particle, which was used as a probe to optically follow cell-signaling pathways in vivo at the single-molecule level. These \^{a}€œcrown nanoparticle plasmon rulers\^{a}€ allowed us to continuously monitor trajectories of caspase-3 activity in live cells for over 2 h, providing sufficient time to observe early-stage caspase-3 activation, which was not possible by conventional ensemble analyses.}, author = {Jun, Young-wook and Sheikholeslami, Sassan and Hostetter, Daniel R. and Tajon, Cheryl and Craik, Charles S. and Alivisatos, A. Paul}, citeulike-article-id = {5977599}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0907367106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/42/17735.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/42/17735.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19805121}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19805121}, day = {20}, doi = {10.1073/pnas.0907367106}, journal = {Proceedings of the National Academy of Sciences}, keywords = {cells, distance, jun09pdf, molecular, molecule, plasmon, resonance, ruler, single, tragectories}, month = {October}, number = {42}, pages = {17735--17740}, posted-at = {2009-10-21 00:41:19}, priority = {2}, title = {Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level}, url = {http://dx.doi.org/10.1073/pnas.0907367106}, volume = {106}, year = {2009} }

TY - JOUR ID - citeulike:5977599 L3 - citeulike-article-id:5977599 N2 - 10.1073/pnas.0907367106 The use of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of “plasmon rulers” to observe conformational changes of single biomolecules in vitro. Plasmon rulers provide robust signals without photobleaching or blinking. Here, we show the first application of plasmon rulers to in vivo studies to observe very long trajectories of single biomolecules in live cells. We present a unique type of plasmon ruler comprised of peptide-linked gold nanoparticle satellites around a core particle, which was used as a probe to optically follow cell-signaling pathways in vivo at the single-molecule level. These “crown nanoparticle plasmon rulers” allowed us to continuously monitor trajectories of caspase-3 activity in live cells for over 2 h, providing sufficient time to observe early-stage caspase-3 activation, which was not possible by conventional ensemble analyses. IS - 42 JF - Proceedings of the National Academy of Sciences EP - 17740 TI - Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level VL - 106 SP - 17735 KW - cells KW - distance KW - jun09pdf KW - molecular KW - molecule KW - plasmon KW - resonance KW - ruler KW - single KW - tragectories AU - Jun, Young-wook AU - Sheikholeslami, Sassan AU - Hostetter, Daniel AU - Tajon, Cheryl AU - Craik, Charles AU - Alivisatos, Paul PY - 2009/10/20/ UR - http://dx.doi.org/10.1073/pnas.0907367106 ER -