Singularity in Polarization: Rewiring Yeast Cells to Make Two Buds Export

@article{citeulike:6106376, abstract = {For budding yeast to ensure formation of only one bud, cells must polarize toward one, and only one, site. Polarity establishment involves the Rho family GTPase Cdc42, which concentrates at polarization sites via a positive feedback loop. To assess whether singularity is linked to the specific Cdc42 feedback loop, we disabled the yeast cell's endogenous amplification mechanism and synthetically rewired the cells to employ a different positive feedback loop. Rewired cells violated singularity, occasionally making two buds. Even cells that made only one bud sometimes initiated two clusters of Cdc42, but then one cluster became dominant. Mathematical modeling indicated that, given sufficient time, competition between clusters would promote singularity. In rewired cells, competition occurred slowly and sometimes failed to develop a single winning cluster before budding. Slowing competition in normal cells also allowed occasional formation of two buds, suggesting that singularity is enforced by rapid competition between Cdc42 clusters.}, author = {Howell, Audrey S. and Savage, Natasha S. and Johnson, Sam A. and Bose, Indrani and Wagner, Allison W. and Zyla, Trevin R. and Nijhout, H. Frederik and Reed, Michael C. and Goryachev, Andrew B. and Lew, Daniel J.}, citeulike-article-id = {6106376}, citeulike-linkout-0 = {http://www.cell.com//abstract/S0092-8674(09)01314-2}, day = {13}, keywords = {cell-decision, modelling, scerevisiae, signaling, systems-biology}, month = {November}, number = {4}, pages = {731--743}, posted-at = {2009-11-12 22:34:23}, priority = {2}, title = {Singularity in Polarization: Rewiring Yeast Cells to Make Two Buds}, url = {http://www.cell.com//abstract/S0092-8674(09)01314-2}, volume = {139}, year = {2009} }

TY - JOUR ID - citeulike:6106376 L3 - citeulike-article-id:6106376 N2 - For budding yeast to ensure formation of only one bud, cells must polarize toward one, and only one, site. Polarity establishment involves the Rho family GTPase Cdc42, which concentrates at polarization sites via a positive feedback loop. To assess whether singularity is linked to the specific Cdc42 feedback loop, we disabled the yeast cell's endogenous amplification mechanism and synthetically rewired the cells to employ a different positive feedback loop. Rewired cells violated singularity, occasionally making two buds. Even cells that made only one bud sometimes initiated two clusters of Cdc42, but then one cluster became dominant. Mathematical modeling indicated that, given sufficient time, competition between clusters would promote singularity. In rewired cells, competition occurred slowly and sometimes failed to develop a single winning cluster before budding. Slowing competition in normal cells also allowed occasional formation of two buds, suggesting that singularity is enforced by rapid competition between Cdc42 clusters. IS - 4 EP - 743 TI - Singularity in Polarization: Rewiring Yeast Cells to Make Two Buds VL - 139 SP - 731 KW - cell-decision KW - modelling KW - scerevisiae KW - signaling KW - systems-biology AU - Howell, Audrey AU - Savage, Natasha AU - Johnson, Sam AU - Bose, Indrani AU - Wagner, Allison AU - Zyla, Trevin AU - Nijhout, Frederik AU - Reed, Michael AU - Goryachev, Andrew AU - Lew, Daniel PY - 2009/11/13/ UR - http://www.cell.com//abstract/S0092-8674(09)01314-2 ER -