The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression Export

@article{citeulike:1414570, abstract = {Pax5 activity is enhanced in activated B cells and is essential for class switch recombination (CSR). We show that inhibitor of differentiation (Id)2 suppresses CSR by repressing the gene expression of activation-induced cytidine deaminase (AID), which has been shown to be indispensable for CSR. Furthermore, a putative regulatory region of AID contains E2A- and Pax5-binding sites, and the latter site is indispensable for AID gene expression. Moreover, the DNA-binding activity of Pax5 is decreased in Id2-overexpressing B cells and enhanced in Id2-/- B cells. The kinetics of Pax5, but not E2A, occupancy to AID locus is the same as AID expression in primary B cells. Finally, enforced expression of Pax5 induces AID transcription in pro-B cell lines. Our results provide evidence that the balance between Pax5 and Id2 activities has a key role in AID gene expression. 10.1084/jem.20030802}, author = {Gonda, Hiroyuki and Sugai, Manabu and Nambu, Yukiko and Katakai, Tomoya and Agata, Yasutoshi and Mori, Kazuhiro J. and Yokota, Yoshifumi and Shimizu, Akira}, citeulike-article-id = {1414570}, citeulike-linkout-0 = {http://dx.doi.org/10.1084/jem.20030802}, citeulike-linkout-1 = {http://www.jem.org/cgi/content/abstract/198/9/1427}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14581609}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14581609}, day = {3}, doi = {10.1084/jem.20030802}, journal = {J. Exp. Med.}, keywords = {amlt821}, month = {November}, number = {9}, pages = {1427--1437}, posted-at = {2007-06-26 23:57:22}, priority = {2}, title = {The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression}, url = {http://dx.doi.org/10.1084/jem.20030802}, volume = {198}, year = {2003} }

TY - JOUR ID - citeulike:1414570 L3 - citeulike-article-id:1414570 N2 - Pax5 activity is enhanced in activated B cells and is essential for class switch recombination (CSR). We show that inhibitor of differentiation (Id)2 suppresses CSR by repressing the gene expression of activation-induced cytidine deaminase (AID), which has been shown to be indispensable for CSR. Furthermore, a putative regulatory region of AID contains E2A- and Pax5-binding sites, and the latter site is indispensable for AID gene expression. Moreover, the DNA-binding activity of Pax5 is decreased in Id2-overexpressing B cells and enhanced in Id2-/- B cells. The kinetics of Pax5, but not E2A, occupancy to AID locus is the same as AID expression in primary B cells. Finally, enforced expression of Pax5 induces AID transcription in pro-B cell lines. Our results provide evidence that the balance between Pax5 and Id2 activities has a key role in AID gene expression. 10.1084/jem.20030802 IS - 9 JF - J. Exp. Med. EP - 1437 TI - The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression VL - 198 SP - 1427 KW - amlt821 AU - Gonda, Hiroyuki AU - Sugai, Manabu AU - Nambu, Yukiko AU - Katakai, Tomoya AU - Agata, Yasutoshi AU - Mori, Kazuhiro AU - Yokota, Yoshifumi AU - Shimizu, Akira PY - 2003/11/03/ UR - http://dx.doi.org/10.1084/jem.20030802 ER -