Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. Export

@article{citeulike:3768618, abstract = {microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated HEK293 cell lines stably expressing epitope-tagged human AGO proteins and other RNA silencing-related proteins and used these cells to purify miRNA-containing RNPs. Mass spectrometric analyses of the proteins associated with different AGO proteins revealed a common set of helicases and mRNA-binding proteins, among them the three trinucleotide repeat containing proteins 6 (TNRC6A,-B,-C). mRNA microarray analyses of these miRNA-associated RNPs revealed that AGO and TNRC6 proteins bind highly similar sets of transcripts enriched in binding sites for highly expressed endogenous miRNAs, indicating that the TNRC6 proteins are a component of the mRNA-targeting miRNA silencing complex. Together with the very similar proteomic composition of each AGO complex, this result suggests substantial functional redundancy within families of human AGO and TNRC6 proteins. Our results further demonstrate that we have developed an effective biochemical approach to identify physiologically relevant human miRNA targets.}, author = {Landthaler, Markus and Gaidatzis, Dimos and Rothballer, Andrea and Chen, Po Yu Y. and Soll, Steven Joseph J. and Dinic, Lana and Ojo, Tolulope and Hafner, Markus and Zavolan, Mihaela and Tuschl, Thomas}, citeulike-article-id = {3768618}, citeulike-linkout-0 = {http://dx.doi.org/10.1261/rna.1351608}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18978028}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18978028}, doi = {10.1261/rna.1351608}, issn = {1469-9001}, journal = {RNA (New York, N.Y.)}, keywords = {biochemical\_method, mechanism, microrna, target}, month = {December}, number = {12}, pages = {2580--2596}, posted-at = {2009-01-12 04:09:06}, priority = {2}, title = {Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs.}, url = {http://dx.doi.org/10.1261/rna.1351608}, volume = {14}, year = {2008} }

TY - JOUR ID - citeulike:3768618 L3 - citeulike-article-id:3768618 N2 - microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated HEK293 cell lines stably expressing epitope-tagged human AGO proteins and other RNA silencing-related proteins and used these cells to purify miRNA-containing RNPs. Mass spectrometric analyses of the proteins associated with different AGO proteins revealed a common set of helicases and mRNA-binding proteins, among them the three trinucleotide repeat containing proteins 6 (TNRC6A,-B,-C). mRNA microarray analyses of these miRNA-associated RNPs revealed that AGO and TNRC6 proteins bind highly similar sets of transcripts enriched in binding sites for highly expressed endogenous miRNAs, indicating that the TNRC6 proteins are a component of the mRNA-targeting miRNA silencing complex. Together with the very similar proteomic composition of each AGO complex, this result suggests substantial functional redundancy within families of human AGO and TNRC6 proteins. Our results further demonstrate that we have developed an effective biochemical approach to identify physiologically relevant human miRNA targets. IS - 12 JF - RNA (New York, N.Y.) SN - 1469-9001 EP - 2596 TI - Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. VL - 14 SP - 2580 KW - biochemical_method KW - mechanism KW - microrna KW - target AU - Landthaler, Markus AU - Gaidatzis, Dimos AU - Rothballer, Andrea AU - Chen, Po Yu AU - Soll, Steven Joseph AU - Dinic, Lana AU - Ojo, Tolulope AU - Hafner, Markus AU - Zavolan, Mihaela AU - Tuschl, Thomas PY - 2008/12// UR - http://dx.doi.org/10.1261/rna.1351608 ER -