Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels. Export

@article{citeulike:1043443, abstract = {We have isolated a novel cDNA, that appears to represent a new class of ion channels, by using the yeast two-hybrid system and the SH3 domain of the neural form of Src (N-src) as a bait. The encoded polypeptide, BCNG-1, is distantly related to cyclic nucleotide-gated channels and the voltage-gated channels, Eag and H-erg. BCNG-1 is expressed exclusively in the brain, as a glycosylated protein of approximately 132 kDa. Immunohistochemical analysis indicates that BCNG-1 is preferentially expressed in specific subsets of neurons in the neocortex, hippocampus, and cerebellum, in particular pyramidal neurons and basket cells. Within individual neurons, the BCNG-1 protein is localized to either the dendrites or the axon terminals depending on the cell type. Southern blot analysis shows that several other BCNG-related sequences are present in the mouse genome, indicating the emergence of an entire subfamily of ion channel coding genes. These findings suggest the existence of a new type of ion channel, which is potentially able to modulate membrane excitability in the brain and could respond to regulation by cyclic nucleotides.}, address = {Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, and Howard Hughes Medical Institute, 722 West 168th Street, New York, NY 10032, USA.}, author = {Santoro, B. and Grant, S. G. and Bartsch, D. and Kandel, E. R.}, citeulike-article-id = {1043443}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9405696}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9405696}, day = {23}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {hcn}, month = {December}, number = {26}, pages = {14815--14820}, posted-at = {2007-01-15 21:10:04}, priority = {2}, title = {Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9405696}, volume = {94}, year = {1997} }

TY - JOUR ID - citeulike:1043443 L3 - citeulike-article-id:1043443 N2 - We have isolated a novel cDNA, that appears to represent a new class of ion channels, by using the yeast two-hybrid system and the SH3 domain of the neural form of Src (N-src) as a bait. The encoded polypeptide, BCNG-1, is distantly related to cyclic nucleotide-gated channels and the voltage-gated channels, Eag and H-erg. BCNG-1 is expressed exclusively in the brain, as a glycosylated protein of approximately 132 kDa. Immunohistochemical analysis indicates that BCNG-1 is preferentially expressed in specific subsets of neurons in the neocortex, hippocampus, and cerebellum, in particular pyramidal neurons and basket cells. Within individual neurons, the BCNG-1 protein is localized to either the dendrites or the axon terminals depending on the cell type. Southern blot analysis shows that several other BCNG-related sequences are present in the mouse genome, indicating the emergence of an entire subfamily of ion channel coding genes. These findings suggest the existence of a new type of ion channel, which is potentially able to modulate membrane excitability in the brain and could respond to regulation by cyclic nucleotides. IS - 26 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, and Howard Hughes Medical Institute, 722 West 168th Street, New York, NY 10032, USA. EP - 14820 TI - Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels. VL - 94 SP - 14815 KW - hcn AU - Santoro, B AU - Grant, SG AU - Bartsch, D AU - Kandel, ER PY - 1997/12/23/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9405696 ER -