Whole-cell recording of the Ca(2+)-dependent slow afterhyperpolarization in hippocampal neurones: effects of internally applied anions. Export

@article{citeulike:2307958, abstract = {Using the whole-cell recording technique, we have examined the slow Ca(2+)-activated afterhyperpolarization (AHP) and its underlying current (IAHP) in hippocampal CA1 neurones of brain slices obtained from mature rats. Specifically we have studied the effects of the anion component of various K+ salts commonly used to make the pipette filling solution that dialyses neurones during whole-cell recordings. Among the K+ salts examined which included potassium methylsulfate, potassium methanesulfonate, potassium gluconate, potassium chloride, potassium citrate and potassium glutamate, stable AHPs/IAHP and strong spike firing adaptation could only be observed in neurones recorded with the patch pipette solution containing potassium methylsulfate. These AHPs and firing patterns closely mimicked those recorded with sharp electrodes. Similarly, the sustained component of voltage-activated Ca2+ currents was more stable in neurones dialysed with cesium methanesulfonate than in those dialysed with cesium gluconate or cesium chloride. Although the mechanisms underlying the interaction(s) between internally applied anions and ionic channels need further investigation, the present experiments illustrate that in mammalian brain neurones at 33 degrees C, the Ca(2+)-activated IAHP is dramatically altered by internal anions. We suggest that among anions commonly used in electrode filling solutions for whole-cell recordings, methylsulfate is the least disruptive to intracellular structures or Ca2+ homeostasis and permits stable whole-cell recording of the IAHP and Ca2+ currents in mammalian CNS neurones.}, address = {Playfair Neuroscience Unit, Bloorview Epilepsy Research Program, Toronto Hospital Research Institute, Toronto Hospital, Ontario, Canada.}, author = {Zhang, L. and Weiner, J. L. and Valiante, T. A. and Velumian, A. A. and Watson, P. L. and Jahromi, S. S. and Schertzer, S. and Pennefather, P. and Carlen, P. L.}, citeulike-article-id = {2307958}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8183635}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8183635}, issn = {0031-6768}, journal = {Pflugers Arch}, keywords = {ahp, caesium, hippocampe}, month = {February}, number = {3-4}, pages = {247--253}, posted-at = {2008-01-30 16:53:45}, priority = {2}, title = {Whole-cell recording of the Ca(2+)-dependent slow afterhyperpolarization in hippocampal neurones: effects of internally applied anions.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8183635}, volume = {426}, year = {1994} }

TY - JOUR ID - citeulike:2307958 L3 - citeulike-article-id:2307958 N2 - Using the whole-cell recording technique, we have examined the slow Ca(2+)-activated afterhyperpolarization (AHP) and its underlying current (IAHP) in hippocampal CA1 neurones of brain slices obtained from mature rats. Specifically we have studied the effects of the anion component of various K+ salts commonly used to make the pipette filling solution that dialyses neurones during whole-cell recordings. Among the K+ salts examined which included potassium methylsulfate, potassium methanesulfonate, potassium gluconate, potassium chloride, potassium citrate and potassium glutamate, stable AHPs/IAHP and strong spike firing adaptation could only be observed in neurones recorded with the patch pipette solution containing potassium methylsulfate. These AHPs and firing patterns closely mimicked those recorded with sharp electrodes. Similarly, the sustained component of voltage-activated Ca2+ currents was more stable in neurones dialysed with cesium methanesulfonate than in those dialysed with cesium gluconate or cesium chloride. Although the mechanisms underlying the interaction(s) between internally applied anions and ionic channels need further investigation, the present experiments illustrate that in mammalian brain neurones at 33 degrees C, the Ca(2+)-activated IAHP is dramatically altered by internal anions. We suggest that among anions commonly used in electrode filling solutions for whole-cell recordings, methylsulfate is the least disruptive to intracellular structures or Ca2+ homeostasis and permits stable whole-cell recording of the IAHP and Ca2+ currents in mammalian CNS neurones. IS - 3-4 JF - Pflugers Arch SN - 0031-6768 AD - Playfair Neuroscience Unit, Bloorview Epilepsy Research Program, Toronto Hospital Research Institute, Toronto Hospital, Ontario, Canada. EP - 253 TI - Whole-cell recording of the Ca(2+)-dependent slow afterhyperpolarization in hippocampal neurones: effects of internally applied anions. VL - 426 SP - 247 KW - ahp KW - caesium KW - hippocampe AU - Zhang, L AU - Weiner, JL AU - Valiante, TA AU - Velumian, AA AU - Watson, PL AU - Jahromi, SS AU - Schertzer, S AU - Pennefather, P AU - Carlen, PL PY - 1994/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/8183635 ER -