Backpropagating Action Potentials Trigger Dendritic Release of BDNF during Spontaneous Network Activity Export

@article{citeulike:2969561, abstract = {Brain-derived neurotrophic factor (BDNF) is a major regulator of activity-dependent synapse development and plasticity. Because BDNF is a secreted protein, it has been proposed that BDNF is released from target neurons in an activity-dependent manner. However, direct evidence for postsynaptic release of BDNF triggered by ongoing network activity is still lacking. Here we transfected cultures of dissociated hippocampal neurons with green fluorescent protein (GFP)-tagged BDNF and combined whole-cell recording, time-lapse fluorescent imaging, and immunostaining to monitor activity-dependent dendritic release of BDNF. We found that spontaneous backpropagating action potentials, but not synaptic activity alone, led to a Ca2+-dependent dendritic release of BDNF-GFP. Moreover, we provide evidence that endogenous BDNF released from a single neuron can phosphorylate CREB (cAMP response element-binding protein) in neighboring neurons, an important step of immediate early gene activation. Therefore, together, our results support the hypothesis that BDNF might act as a target-derived messenger of activity-dependent synaptic plasticity and development. 10.1523/JNEUROSCI.1673-08.2008}, author = {Kuczewski, Nicola and Porcher, Christophe and Ferrand, Nadine and Fiorentino, Herve and Pellegrino, Christophe and Kolarow, Richard and Lessmann, Volkmar and Medina, Igor and Gaiarsa, Jean-Luc}, citeulike-article-id = {2969561}, citeulike-linkout-0 = {http://dx.doi.org/10.1523/JNEUROSCI.1673-08.2008}, citeulike-linkout-1 = {http://www.jneurosci.org/cgi/content/abstract/28/27/7013}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18596175}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18596175}, day = {2}, doi = {10.1523/JNEUROSCI.1673-08.2008}, journal = {J. Neurosci.}, keywords = {backpropagatingpa, bdnf, stdp}, month = {July}, number = {27}, pages = {7013--7023}, posted-at = {2008-09-03 12:53:10}, priority = {2}, title = {Backpropagating Action Potentials Trigger Dendritic Release of BDNF during Spontaneous Network Activity}, url = {http://dx.doi.org/10.1523/JNEUROSCI.1673-08.2008}, volume = {28}, year = {2008} }

TY - JOUR ID - citeulike:2969561 L3 - citeulike-article-id:2969561 N2 - Brain-derived neurotrophic factor (BDNF) is a major regulator of activity-dependent synapse development and plasticity. Because BDNF is a secreted protein, it has been proposed that BDNF is released from target neurons in an activity-dependent manner. However, direct evidence for postsynaptic release of BDNF triggered by ongoing network activity is still lacking. Here we transfected cultures of dissociated hippocampal neurons with green fluorescent protein (GFP)-tagged BDNF and combined whole-cell recording, time-lapse fluorescent imaging, and immunostaining to monitor activity-dependent dendritic release of BDNF. We found that spontaneous backpropagating action potentials, but not synaptic activity alone, led to a Ca2+-dependent dendritic release of BDNF-GFP. Moreover, we provide evidence that endogenous BDNF released from a single neuron can phosphorylate CREB (cAMP response element-binding protein) in neighboring neurons, an important step of immediate early gene activation. Therefore, together, our results support the hypothesis that BDNF might act as a target-derived messenger of activity-dependent synaptic plasticity and development. 10.1523/JNEUROSCI.1673-08.2008 IS - 27 JF - J. Neurosci. EP - 7023 TI - Backpropagating Action Potentials Trigger Dendritic Release of BDNF during Spontaneous Network Activity VL - 28 SP - 7013 KW - backpropagatingpa KW - bdnf KW - stdp AU - Kuczewski, Nicola AU - Porcher, Christophe AU - Ferrand, Nadine AU - Fiorentino, Herve AU - Pellegrino, Christophe AU - Kolarow, Richard AU - Lessmann, Volkmar AU - Medina, Igor AU - Gaiarsa, Jean-Luc PY - 2008/07/02/ UR - http://dx.doi.org/10.1523/JNEUROSCI.1673-08.2008 ER -