Polarization sensitive, three-dimensional, single-molecule imaging of cells with a ?double-helix system Export

@article{citeulike:6018989, abstract = {Double-helix point spread function photoactivation-localization microscopy allows three-dimensional (3D) superresolution imaging of objects smaller than the optical diffraction-limit. We demonstrate polarization sensitive detection with 3D super-localization of single-molecules and unveil 3D polarization specific characteristics of single-molecules within the intracellular structure of PtK1 cells expressing photoactivatable green fluorescent protein. The system modulates orthogonal polarization components of single-molecule emissions with a single spatial light modulator and detects them separately with a single detector. Information obtained from the two polarization channels demonstrates polarization based contrast in 3D superresolution imaging. Further, we show that the 3D information from the two channels can be optimally combined to yield up to 30\% improvement in localization precision relative to a single polarization channel system.}, author = {Pavani, Sri R. and DeLuca, Jennifer G. and Piestun, Rafael}, citeulike-article-id = {6018989}, citeulike-linkout-0 = {http://dx.doi.org/10.1364/OE.17.019644}, citeulike-linkout-1 = {http://www.opticsinfobase.org/abstract.cfm?id=186846}, day = {26}, doi = {10.1364/OE.17.019644}, journal = {Opt. Express}, keywords = {single\_molecule, super-resolution}, month = {October}, number = {22}, pages = {19644--19655}, posted-at = {2009-10-28 13:21:19}, priority = {2}, publisher = {OSA}, title = {Polarization sensitive, three-dimensional, single-molecule imaging of cells with a ?double-helix system}, url = {http://dx.doi.org/10.1364/OE.17.019644}, volume = {17}, year = {2009} }

TY - JOUR ID - citeulike:6018989 L3 - citeulike-article-id:6018989 N2 - Double-helix point spread function photoactivation-localization microscopy allows three-dimensional (3D) superresolution imaging of objects smaller than the optical diffraction-limit. We demonstrate polarization sensitive detection with 3D super-localization of single-molecules and unveil 3D polarization specific characteristics of single-molecules within the intracellular structure of PtK1 cells expressing photoactivatable green fluorescent protein. The system modulates orthogonal polarization components of single-molecule emissions with a single spatial light modulator and detects them separately with a single detector. Information obtained from the two polarization channels demonstrates polarization based contrast in 3D superresolution imaging. Further, we show that the 3D information from the two channels can be optimally combined to yield up to 30% improvement in localization precision relative to a single polarization channel system. IS - 22 JF - Opt. Express EP - 19655 TI - Polarization sensitive, three-dimensional, single-molecule imaging of cells with a ?double-helix system PB - OSA VL - 17 SP - 19644 KW - single_molecule KW - super-resolution AU - Pavani, Sri AU - DeLuca, Jennifer AU - Piestun, Rafael PY - 2009/10/26/ UR - http://dx.doi.org/10.1364/OE.17.019644 ER -